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Research Article | Host-Microbe Biology

Four-Dimensional Characterization of the Babesia divergens Asexual Life Cycle, from the Trophozoite to the Multiparasite Stage

José Javier Conesa, Elena Sevilla, María Carmen Terrón, Luis Miguel González, Jeremy Gray, Ana J. Pérez-Berná, José L. Carrascosa, Eva Pereiro, Francisco Javier Chichón, Daniel Luque, Estrella Montero
Ira J. Blader, Editor
José Javier Conesa
aDepartment of Structure of Macromolecules, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Madrid, Spain
bMistral Beamline, Experiment Division, ALBA Synchrotron (ALBA-CELLS), Barcelona, Spain
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Elena Sevilla
cParasitology Reference and Research Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
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María Carmen Terrón
dElectron and Confocal Microscopy Unit, Unidades Centrales Científico-Técnicas (SG-SAFI), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
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Luis Miguel González
cParasitology Reference and Research Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
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Jeremy Gray
eUCD School of Biology and Environmental Science, University College Dublin, Dublin, Ireland
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Ana J. Pérez-Berná
bMistral Beamline, Experiment Division, ALBA Synchrotron (ALBA-CELLS), Barcelona, Spain
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José L. Carrascosa
aDepartment of Structure of Macromolecules, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Madrid, Spain
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Eva Pereiro
bMistral Beamline, Experiment Division, ALBA Synchrotron (ALBA-CELLS), Barcelona, Spain
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Francisco Javier Chichón
aDepartment of Structure of Macromolecules, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Madrid, Spain
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Daniel Luque
dElectron and Confocal Microscopy Unit, Unidades Centrales Científico-Técnicas (SG-SAFI), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
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Estrella Montero
cParasitology Reference and Research Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
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Ira J. Blader
University at Buffalo
Roles: Editor
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DOI: 10.1128/mSphere.00928-20
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Article Information

DOI 
https://doi.org/10.1128/mSphere.00928-20
PubMed 
33055261

Published By 
American Society for Microbiology Journals
History 
  • Received September 24, 2020
  • Accepted September 25, 2020
  • Published online October 14, 2020.

Copyright & Usage 
Copyright © 2020 Conesa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Ira J. Blader, Editor, University at Buffalo

Author Information

  1. José Javier Conesaa,b,
  2. Elena Sevillac,
  3. María Carmen Terrónd,
  4. Luis Miguel Gonzálezc,
  5. Jeremy Graye,
  6. Ana J. Pérez-Bernáb,
  7. José L. Carrascosaa,
  8. Eva Pereirob,
  9. Francisco Javier Chichóna,
  10. Daniel Luqued and
  11. Estrella Monteroc
  1. aDepartment of Structure of Macromolecules, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Madrid, Spain
  2. bMistral Beamline, Experiment Division, ALBA Synchrotron (ALBA-CELLS), Barcelona, Spain
  3. cParasitology Reference and Research Laboratory, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
  4. dElectron and Confocal Microscopy Unit, Unidades Centrales Científico-Técnicas (SG-SAFI), Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
  5. eUCD School of Biology and Environmental Science, University College Dublin, Dublin, Ireland
  • Address correspondence to Daniel Luque, dluque{at}isciii.es, or Estrella Montero, estrella.montero{at}isciii.es.
  • José Javier Conesa and Elena Sevilla contributed equally to this article. Author order was determined on the basis of seniority.

  • Citation Conesa JJ, Sevilla E, Terrón MC, González LM, Gray J, Pérez-Berná AJ, Carrascosa JL, Pereiro E, Chichón FJ, Luque D, Montero E. 2020. Four-dimensional characterization of the Babesia divergens asexual life cycle, from the trophozoite to the multiparasite stage. mSphere 5:e00928-20. https://doi.org/10.1128/mSphere.00928-20.

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Four-Dimensional Characterization of the Babesia divergens Asexual Life Cycle, from the Trophozoite to the Multiparasite Stage
José Javier Conesa, Elena Sevilla, María Carmen Terrón, Luis Miguel González, Jeremy Gray, Ana J. Pérez-Berná, José L. Carrascosa, Eva Pereiro, Francisco Javier Chichón, Daniel Luque, Estrella Montero
mSphere Oct 2020, 5 (5) e00928-20; DOI: 10.1128/mSphere.00928-20

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Four-Dimensional Characterization of the Babesia divergens Asexual Life Cycle, from the Trophozoite to the Multiparasite Stage
José Javier Conesa, Elena Sevilla, María Carmen Terrón, Luis Miguel González, Jeremy Gray, Ana J. Pérez-Berná, José L. Carrascosa, Eva Pereiro, Francisco Javier Chichón, Daniel Luque, Estrella Montero
mSphere Oct 2020, 5 (5) e00928-20; DOI: 10.1128/mSphere.00928-20
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KEYWORDS

Babesia divergens
cryo-soft X-ray tomography
intraerythrocytic asexual cycle
pathogen-host cell interactions
time-lapse video microscopy

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