Article Figures & Data
Figures
- FIG 1
Type II-A CRISPR-Cas system of S. mutans P42S. Squences are from 5′ to 3′. The tracrRNA is in yellow, the cas genes are in blue, the leader is a black arrow, the repeats are represented as red diamonds, and the spacers are green squares.
- FIG 2
tracrRNA in S. mutans. (A) Comparison of predicted tracrRNA in S. mutans UA159 and P42S. Complementarity to crRNA is highlighted in green. (B) The anti-repeat region within the tracrRNA of S. mutans UA159 and P42S compared to crRNA.
- FIG 3
PAM downstream of protospacers.
- FIG 4
Percent identity between Cas9 N terminus and C terminus found in several S. mutans strains.
Tables
- TABLE 1
Relative frequencies of acquired PAMs
FIG S1
Comparison of cas genes (left) and Cas proteins (right) of nine S. mutans strains. Percent identity to the sequences found in S. mutans P42S is indicated. Download FIG S1, TIF file, 0.3 MB.
Copyright © 2020 Mosterd and Moineau.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S1
List of spacers acquired by different bacteriophage insensitive mutants of S. mutans. Mismatches to the M102AD genome are shown in italics in the protospacer sequence. Spacers that are not 100% identical to M102AD are indicated by an asterisk in the spacer column. Download Table S1, DOCX file, 0.02 MB.
Copyright © 2020 Mosterd and Moineau.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S2
Construct insert sequences. Protospacer sequences with flanking PAM are in italics. Download Table S2, DOCX file, 0.02 MB.
Copyright © 2020 Mosterd and Moineau.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
