Research Article | Host-Microbe Biology

The Galleria mellonella Infection Model Does Not Accurately Differentiate between Hypervirulent and Classical Klebsiella pneumoniae

Thomas A. Russo, Ulrike MacDonald
Christopher J. Papasian, Editor
DOI: 10.1128/mSphere.00850-19

Article Figures & Data

Figures

  • FIG 1
    FIG 1

    Mortality in the CD1 murine infection models after challenge with the hvKp and cKp strain cohorts. Each symbol represents the mean mortality for a given strain over time. n = 25 for each strain cohort. (A) Day 1 to day 14 mortality after challenge with 2 × 103 to 5 × 103 CFU of both the hvKp and cKp strain cohorts. For the hvKp strain cohort, means ± standard deviations (SD) are depicted. (B) Murine model: day 1 to day 14 mortality after challenge with the cKp strain cohort at challenge inocula of 3 × 107 to 6 × 107 CFU. Animals were not challenged with the hvKp strain cohort at this challenge inoculum due to the predicted lethal result. *, P < 0.05 (2-way ANOVA with a Bonferroni posttest).

  • FIG 2
    FIG 2

    Mortality in the Galleria mellonella infection model after challenge with the hvKp and cKp strain cohorts at inocula of 1 × 103 to 5 × 103 CFU. Each symbol represents the mean mortality for a given strain. n = 25 for each strain cohort. (A) Day 1. (B) Day 2. (C) Day 3. (D) Day 4. (E) Day 5. (F) Days 1 to 5. For panels A to E, means ± SD are depicted; for panel F, the horizontal line depicts the mean.

  • FIG 3
    FIG 3

    Mortality in the Galleria mellonella infection model after challenge with the hvKp and cKp strain cohorts at inocula of 1 × 104 to 5 × 104 CFU. Each symbol represents the mean mortality for a given strain. n = 25 for each strain cohort. (A) Day 1. (B) Day 2. (C) Day 3. (D) Day 4. (E) Day 5. (F) Days 1 to 5. For panels A to E, means ± SD are depicted; for panel F, the horizontal line depicts the mean. *, P < 0.05 (2-way ANOVA with a Bonferroni posttest).

  • FIG 4
    FIG 4

    Mortality in the Galleria mellonella infection model after challenge with hvKp and cKp strain cohort inocula of 1 × 105 to 5 × 105 CFU. Each symbol represents the mean mortality for a given strain. n = 25 for each strain cohort. (A) Day 1. (B) Day 2. (C) Day 3. (D) Day 4. (E) Day 5. (F) Days 1 to 5. For panels A to E, means ± SD are depicted; for panel F, the horizontal line depicts the mean. *, P < 0.05 (2-way ANOVA with a Bonferroni posttest).

Tables

  • TABLE 1

    Daily percent mortality in the Galleria mellonella infection model

    TABLE 1

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KEYWORDS

Galleria mellonella infection model
classical Klebsiella pneumoniae
hypervirulent Klebsiella pneumoniae
infection models
outbred mouse systemic infection model
virulence

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