Skip to main content
  • ASM Journals
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Latest Articles
    • COVID-19 Research and News from ASM Journals
    • mSphere of Influence: Commentaries from Early Career Microbiologists
    • Archive
  • Topics
    • Applied and Environmental Science
    • Clinical Science and Epidemiology
    • Ecological and Evolutionary Science
    • Host-Microbe Biology
    • Molecular Biology and Physiology
    • Therapeutics and Prevention
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About mSphere
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • ASM Journals
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
mSphere
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Latest Articles
    • COVID-19 Research and News from ASM Journals
    • mSphere of Influence: Commentaries from Early Career Microbiologists
    • Archive
  • Topics
    • Applied and Environmental Science
    • Clinical Science and Epidemiology
    • Ecological and Evolutionary Science
    • Host-Microbe Biology
    • Molecular Biology and Physiology
    • Therapeutics and Prevention
  • For Authors
    • Getting Started
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About mSphere
    • Editor in Chief
    • Board of Editors
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
Editor's Pick Research Article | Host-Microbe Biology

Manganese Uptake, Mediated by SloABC and MntH, Is Essential for the Fitness of Streptococcus mutans

Jessica K. Kajfasz, Callahan Katrak, Tridib Ganguly, Jonathan Vargas, Logan Wright, Zachary T. Peters, Grace A. Spatafora, Jacqueline Abranches, José A. Lemos
Sarah E. F. D'Orazio, Editor
Jessica K. Kajfasz
aDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Callahan Katrak
aDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tridib Ganguly
aDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan Vargas
aDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Logan Wright
bDepartment of Biology, Middlebury College, Middlebury, Vermont, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zachary T. Peters
bDepartment of Biology, Middlebury College, Middlebury, Vermont, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Grace A. Spatafora
bDepartment of Biology, Middlebury College, Middlebury, Vermont, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jacqueline Abranches
aDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
José A. Lemos
aDepartment of Oral Biology, University of Florida College of Dentistry, Gainesville, Florida, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sarah E. F. D'Orazio
University of Kentucky
Roles: Editor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/mSphere.00764-19
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

ABSTRACT

Early epidemiological studies implicated manganese (Mn) as a possible caries-promoting agent, while laboratory studies have indicated that manganese stimulates the expression of virulence-related factors in the dental pathogen Streptococcus mutans. To better understand the importance of manganese homeostasis to S. mutans pathophysiology, we first used RNA sequencing to obtain the global transcriptional profile of S. mutans UA159 grown under Mn-restricted conditions. Among the most highly expressed genes were those of the entire sloABC operon, encoding a dual iron/manganese transporter, and an uncharacterized gene, here mntH, that codes for a protein bearing strong similarity to Nramp-type transporters. While inactivation of sloC, which encodes the lipoprotein receptor of the SloABC system, or of mntH alone had no major consequence for the overall fitness of S. mutans, simultaneous inactivation of sloC and mntH (ΔsloC ΔmntH) impaired growth and survival under Mn-restricted conditions, including in human saliva or in the presence of calprotectin. Further, disruption of Mn transport resulted in diminished stress tolerance and reduced biofilm formation in the presence of sucrose. These phenotypes were markedly improved when cells were provided with excess Mn. Metal quantifications revealed that the single mutant strains contained intracellular levels of Mn similar to those seen with the parent strain, whereas Mn was nearly undetectable in the ΔsloC ΔmntH strain. Collectively, these results reveal that SloABC and MntH work independently and cooperatively to promote cell growth under Mn-restricted conditions and that maintenance of Mn homeostasis is essential for the expression of major virulence attributes in S. mutans.

IMPORTANCE As transition biometals such as manganese (Mn) are essential for all forms of life, the ability to scavenge biometals in the metal-restricted host environment is an important trait of successful cariogenic pathobionts. Here, we showed that the caries pathogen Streptococcus mutans utilizes two Mn transport systems, namely, SloABC and MntH, to acquire Mn from the environment and that the ability to maintain the cellular levels of Mn is important for the manifestation of characteristics that associate S. mutans with dental caries. Our results indicate that the development of strategies to deprive S. mutans of Mn hold promise in the combat against this important bacterial pathogen.

INTRODUCTION

Transition metals are essential for all domains of life by serving as structural and catalytic cofactors, with approximately 50% of all enzymes in cells requiring a metal cofactor for proper function (1). During microbial infections, the ability of the invading pathogen to acquire iron (Fe), manganese (Mn), and zinc (Zn) becomes particularly relevant as the host employs several mechanisms to sequester these essential biometals as part of an active response known as nutritional immunity (2–5). Specifically, Fe-binding proteins such as transferrin (in serum) and lactoferrin (in secretions) are produced by the host to chelate Fe, thereby restricting its bioavailability to invading pathogens. Similarly, transition metals are actively sequestered by calprotectin, a heterodimeric S100 family protein that is an important part of the inflammatory response during infection, was named for its role in innate immunity, and constitutes about 60% of the total proteins in neutrophils (3, 6, 7). To overcome this micronutrient limitation, bacteria evolved a number of mechanisms for metal acquisition, including the production of low-molecular-weight molecules (metallophores) for extracellular metal capture and of high-affinity membrane-associated metal transporters, as well as tools for direct acquisition of metal from host molecules and proteins (metal piracy) (5).

Streptococcus mutans is regarded as a keystone pathogen in dental caries due to its ability to change the architecture and environment of oral biofilm such that it fosters the outgrowth of acidogenic and aciduric species (such as Lactobacillus spp., Actinomyces spp., Bifidobacterium spp., Scardovia wiggsiae, Streptococcus sobrinus, and S. mutans itself) at the expense of the commensal bacteria associated with oral health (8, 9). The cariogenic potential of S. mutans resides in its ability to (i) form robust biofilms on tooth surfaces in a sucrose-dependent manner; (ii) produce and tolerate large amounts of lactic acid, the major end product of its fermentative metabolism; and (iii) cope with the oxidative stress that arises from the environmental reduction of oxygen and the production of hydrogen peroxide (H2O2) by competing neighbor species (10). In addition to dental caries, S. mutans is also one of the causative agents of infective endocarditis, a life-threatening bacterial infection of the endocardium (11).

Previous studies conducted during the 1970s and 1980s indicated a possible relationship between biometal availability in the oral cavity and caries incidence (12–16). In particular, high rates of caries were linked to elevated levels of Mn in drinking water (12, 14, 16). Despite the existence of conflicting clinical data questioning this correlation (13, 15), few studies have directly investigated the significance of Mn in the pathophysiology of oral streptococci (17–25). An early study aiming to determine the trace element requirement of oral streptococci concluded that Mn was the only trace metal absolutely required for the growth of cariogenic and noncariogenic streptococci in the laboratory setting (22), a finding that was later confirmed by a second group of investigators (17). In addition, Mn was shown to stimulate dextran-dependent aggregation in Streptococcus criceti (formerly S. cricetus) (26), a trait that was found to be mediated by surface-associated glucan-binding proteins (GBPs) and to be critical to sucrose-dependent adhesion and biofilm formation (27). Subsequent studies using both S. criceti and Streptococcus sobrinus strains showed that metal chelating agents such as citrate or EDTA reversibly inhibit glucan-induced aggregation, thereby preventing sucrose-dependent adhesion (24). In addition, confocal microscopy analysis of S. mutans UA159 biofilms grown in the presence of sucrose revealed that Mn-depleted biofilms formed large cell clumps that were more easily washed away than biofilms formed under Mn-replete conditions (18). Manganese was also shown to stimulate carbohydrate metabolism in S. mutans, in particular, the synthesis of glycogen-like intracellular polysaccharide (IPS) stores (21). Finally, when added to drinking water, Mn was shown to increase the cariogenic potential of S. mutans in a germfree rat model (21). It should also be noted that Mn is known to play an important role in the oxidative stress responses of lactic acid bacteria by directly interacting with and scavenging superoxide radicals, by serving as the enzymatic cofactor of the superoxide dismutase (SOD) enzyme, and by replacing Fe as an enzymatic cofactor, thereby protecting Fe-binding proteins from the irreversible damage of Fenton chemistry (28, 29). Collectively, the picture that emerges from these studies is that Mn may serve as a caries-promoting agent by stimulating bacterial metabolism, by facilitating sucrose-dependent biofilm formation, and possibly by conferring protection against the oxidative stresses encountered in dental plaque.

Because the nutrients available in the oral cavity derive, in large part, from the diet, the concentration of Mn in human saliva has been shown to fluctuate from as low as 1 μM (13, 15) to as high as 36 μM (30). Taking into consideration that the concentration of Mn is restricted to the nanomolar range in plasma (31), the concentration of Mn in saliva is unlikely to be a growth-limiting factor for most oral bacteria. And yet, fluctuations in Mn levels may serve as a cue for S. mutans to sense the environment and adjust its metabolism accordingly by favoring a biofilm survival mode over an active-growth mode and/or dispersion mode. Beyond the oral environment, the ability to scavenge Mn in environments in which availability of this metal is known to be restricted, such as the bloodstream and internal organs, has proven to be an essential trait for bacterial pathogens. In fact, a growing number of Mn transport systems have been identified as major virulence factors, including examples where loss of Mn transporters rendered organisms closely related to S. mutans, such as Streptococcus pneumoniae and Enterococcus faecalis, virtually avirulent in animal infection models (32, 33). In S. mutans, previous characterization of pathways associated with Mn homeostasis has been restricted to the metalloregulator SloR and the ABC-type transporter SloABC (34–38). Those studies revealed that specific binding to Fe or Mn triggered function of SloR as a global transcriptional repressor, which includes repression of the sloABC operon (35–37). SloABC was shown to function as a dual Fe and Mn transporter, and the virulence of a sloA mutant strain was attenuated in a rat model of endocarditis (38).

To further our understanding of the significance of Mn homeostasis for S. mutans pathobiology, we first used RNA deep sequencing (RNA-Seq) to compare the transcriptomes of S. mutans serotype c strain UA159 grown in a chemically defined medium under Mn-depleted and Mn-replete conditions. Among the genes highly upregulated during Mn starvation were all genes of the sloABC operon and S. mutans 770c (smu770c), here mntH, coding for a putative metal transporter from the natural resistance-associated macrophage protein-type (Nramp) family. While inactivation of sloC, coding for the SloC lipoprotein receptor, or of mntH alone did not cause a significant impact in the overall fitness of S. mutans, simultaneous inactivation of sloC and mntH (ΔsloC ΔmntH strain) resulted in a dramatic reduction in cellular Mn levels and impaired growth and survival when cells were grown under Mn-restricted conditions. Further characterization of the ΔsloC, ΔmntH, and ΔsloC ΔmntH strains revealed that Mn transport contributes to the ability of S. mutans to cope with acid and oxidative stresses and to form biofilms in the presence of sucrose. Collectively, the data from this study reveal that Mn transport in S. mutans is primarily mediated by SloABC and MntH and support the idea that Mn plays a critical role in the expression of virulence attributes by this important human pathogen.

RESULTS

Transcriptome analysis reveals a new Mn transporter in S. mutans.Comparison of the transcriptome profiles of UA159 grown to mid-exponential phase in a chemically defined medium depleted for Mn (∼0.2 μM Mn) versus growth under Mn-replete (∼130 μM Mn) conditions identified 95 differentially expressed genes (Table 1) (false-discovery rate [FDR] of 0.01, 2-fold cutoff). Among those, 33 genes were upregulated and 62 were downregulated. To ensure that these gene expression trends were indeed due to Mn restriction, the intracellular Mn content of S. mutans UA159 grown under Mn-replete or Mn-depleted conditions was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The analysis confirmed that intracellular Mn content was severely diminished when S. mutans UA159 was grown in the Mn-depleted FMC medium (Fig. 1A).

View this table:
  • View inline
  • View popup
  • Download powerpoint
TABLE 1

S. mutans genes differentially expressed when grown in FMC depleted of Mn compared to FMC complete media

FIG 1
  • Open in new tab
  • Download powerpoint
FIG 1

Summary of RNA-Seq analysis comparing S. mutans UA159 grown under Mn-depleted versus Mn-replete conditions. S. mutans UA159 was grown to an OD600 of 0.4 in FMC medium (complete or depleted of Mn). Total RNA was isolated, and the levels of gene expression under each condition were compared via RNA-Seq analysis. (A) Intracellular Mn content of S. mutans UA159 grown to an OD600 of ∼0.4 in FMC medium (complete or depleted of Mn). The bar graphs show averages and standard deviations of results from five independent ICP-OES analyses. Student's t test was used to compare levels of metal content between the two media (*, P ≤ 0.005). (B) Dot plot of genes differentially expressed under conditions of Mn depletion as determined by Degust (degust.erc.monash.edu). The y axis indicates the log2 fold change in expression compared to control cultures (FMC complete), while the x axis indicates the average expression level of each gene compared to all other genes. The identities of selected genes of interest are indicated. (C) Graphical representations of the functional categories for upregulated or downregulated genes shown in panel B. Biosyn, biosynthesis.

The differentially expressed genes were grouped into 11 functional categories (Fig. 1B and C), with genes encoding transport and binding, DNA metabolism, and hypothetical proteins highly represented in the list of upregulated genes. In contrast, genes encoding hypothetical proteins accounted for more than 50% of the downregulated genes followed by genes involved in transport and binding. The genes that were most highly upregulated during growth under Mn-restricted conditions were those of the dual Fe and Mn transporter sloABC operon (≥56-fold to 99-fold), a small open reading frame (smu185; 71-fold) with the first 18 nucleotides overlapping the sloC gene 3′ end, the sloR transcriptional repressor (16-fold), and the uncharacterized smu770c gene (6-fold) (Table 1; see also Fig. 1B). BLAST search analysis revealed that the protein encoded by smu770c belongs to the Nramp-type transport family predicted to function in metal uptake. The Smu770c protein shared 76% identity with S. agalactiae (group B Streptococcus) MntH and 60% and 54% identity with E. faecalis MntH1 and MntH2 proteins, respectively. Of note, S. agalactiae MntH and E. faecalis MntH1 and MntH2 have been recently assigned a role in Mn uptake (32, 39). Other genes upregulated in the absence of Mn were several belonging to the CRISPR2-cas operon (smu1753c to smu1764c; >4-fold) as well as 3 of 4 genes of the smu995 to smu998 operon (>2-fold), recently shown to code for an Fe transport system (40).

The genes that were found to be most highly repressed when S. mutans was grown under Mn-restricted conditions were a cluster of genes encoding possible conjugative transposon proteins (smu191c to smu217c; ≥2.4-fold downregulated). Additionally, genes encoding proteins with predicted roles in amino acid transport (smu932 to smu936), purine biosynthesis (smu29 to smu32), fatty acid biosynthesis (smu1334c to smu1338c), production of antimicrobial compounds (smu1339c to smu1343c), and sugar transport and metabolism (ftf, smu2028, gtfD, smu910) showed decreased levels of expression under Mn-depleted conditions (Table 1).

SloABC and MntH are the principal manganese transporters in S. mutans.Because of the high degree of conservation between Smu770c and previously characterized MntH proteins from other Firmicutes, we assigned the name “mntH” to the monocistronic transcriptional unit smu770c. Here, we sought to characterize the mntH gene and investigate the possible cooperative nature of SloABC and MntH in metal acquisition. To accomplish this, we created strains bearing single deletions in sloC (ΔsloC), which encodes the metal binding lipoprotein of the SloABC system, or in mntH (ΔmntH), as well as a double mutant strain lacking both sloC and mntH (ΔsloC ΔmntH). All mutant strains were initially isolated on brain heart infusion (BHI) agar supplemented with 75 μM Mn. Upon genetic confirmation of the single and double mutants, we tested the ability of these strains to grow in BHI agar and found that the ΔsloC ΔmntH double mutant was unable to grow on BHI agar without Mn supplementation (Fig. 2A). The ΔsloC ΔmntH strain was able to grow in BHI broth, albeit at much lower rates than the other strains, reaching similar final growth yields after 16 h (Fig. 2B). Supplementation of BHI agar with 25 μM Mn (BHI+Mn) fully restored the growth defect of the double mutant strain in broth (Fig. 2C). We suspected that the different growth behaviors of the ΔsloC ΔmntH strain in BHI plates and in broth were due to trace amounts of Mn that had transferred from the overnight BHI inoculum that contained 7 μM Mn. This suspicion was then confirmed by findings showing that the ΔsloC ΔmntH strain could not grow in unsupplemented BHI agar after a second passage (data not shown). To assess the metal requirements of the mutant strains in a more controlled fashion, growth of the parent UA159 and mutant strains was also monitored in the chemically defined FMC medium (Fe and Mn replete; Table 2) and in FMC medium depleted of Mn (Mn < 90 nM) or Fe (Fe < 90 nM) or both (27). In complete FMC medium, growth of all mutant strains was indistinguishable from that of the parent strain (Fig. 2D). As expected, the ΔsloC ΔmntH double mutant strain failed to grow in Mn-depleted FMC medium whereas the ΔsloC mutant showed a slight growth delay that did not affect the final growth yields (Fig. 2E). Iron depletion alone did not affect growth of the parent strain or of any of the mutant strains, but simultaneous depletion of Fe and Mn exacerbated the slow-growth defect of the ΔsloC strain (Fig. 2F and G). Growth of the ΔsloC ΔmntH strain in plain BHI agar or in Mn-depleted FMC medium was fully restored by complementation when either the sloC or mntH gene was integrated elsewhere in the chromosome (Fig. 2A and H).

FIG 2
  • Open in new tab
  • Download powerpoint
FIG 2

SloABC and MntH promote growth of S. mutans in Mn-depleted environments. (A) Growth of S. mutans UA159 and ΔsloC, ΔmntH, and ΔsloC ΔmntH mutant strains along with the double mutant strain complemented with either sloC or mntH to mid-logarithmic phase (OD600 of ∼0.4) on BHI agar. Overnight cultures were spotted onto BHI agar with or without supplementation with 10 μM Mn. Plates were incubated for 48 h before image was obtained. (B to G) Growth of UA159, ΔsloC, ΔmntH, and ΔsloC ΔmntH mutant strains in (B) BHI broth, (C) BHI broth supplemented with 75 μM Mn, (D) FMC complete (130 μM Mn), (E) Mn-depleted FMC, (F) Fe-depleted FMC, and (G) Mn- and Fe-depleted FMC. (H) Genetic complementation of the ΔsloC ΔmntH growth defect in Mn-depleted FMC with either sloC or mntH. The graphs show averages and standard deviations of results from at least three independent experiments.

View this table:
  • View inline
  • View popup
  • Download powerpoint
TABLE 2

Metal content of media used for growth of S. mutansa

Next, we used ICP-OES to determine the cellular metal content of the parent and mutant strains grown to mid-exponential phase in BHI broth (Fig. 3). Despite not showing a growth defect in plain BHI, the ΔsloC and ΔmntH single mutant strains carried ∼45% less cellular Mn than UA159. In agreement with the results shown in Fig. 2, combined deletion of sloC and mntH resulted in a more significant (∼80%) reduction in cellular Mn pools. Complementation of strain ΔsloC ΔmntH with either one of the inactivated genes restored cellular Mn content to parent strain levels. Despite the previously assigned role of SloABC in Fe uptake (38), intracellular quantities of Fe did not differ significantly among the strains. Likewise, no important differences in intracellular zinc content were observed among the strains (Fig. 2). Collectively, these results reveal that SloABC and MntH comprise the principal Mn transport systems of S. mutans, working cooperatively to maintain Mn homeostasis.

FIG 3
  • Open in new tab
  • Download powerpoint
FIG 3

SloABC and MntH are the main Mn transporters in S. mutans UA159. The bar graph indicates the intracellular manganese, iron, and zinc content of S. mutans UA159 and derivatives grown in plain BHI agar to an OD600 of ∼0.4. Data represent averages and standard deviations of results from five independent ICP-OES analyses. Student's t test was used to compare the metal content of the mutant strains to that of UA159 (*, P ≤ 0.05) and of the double mutant ΔsloC ΔmntH (ΔΔ) to that of the complemented strains (#, P ≤ 0.0005).

mntH is a new member of the SloR regulon.Transcriptional repression of the sloABC operon exerted by SloR has been thoroughly characterized by one of our laboratories (35, 36, 41). A conserved SloR-binding palindrome was identified upstream of the mntH gene in one of those studies (36), but the specificity of SloR binding to the mntH promoter region was not explored at that time. Here, we used quantitative real-time PCR (qRT-PCR) and an electrophoretic mobility shift assay (EMSA) to determine the SloR-mntH relationship. Compared to the parent strain, inactivation of sloR (ΔsloR strain) resulted in ∼5-fold-increased mntH transcription and inactivation of sloA, the first gene of the sloABC operon, in ∼15-fold-increased transcription (Fig. 4A). In addition, EMSAs revealed that a concentration of as low as 60 nM purified SloR shifted mntH probe migration (Fig. 4B) and that the region possibly harbors more than a single SloR binding site given the supershift that was observed with 300 nM SloR. The specificity of SloR binding to the mntH probe was confirmed by showing that addition of the metal chelator EDTA or of excess cold probe disrupted the interaction in a concentration-dependent manner (Fig. 4B). The region upstream of the translational start of mntH includes a pair of hexamers composing a predicted SloR recognition element (SRE) (Fig. 4C), fitting well with the model of SloR binding that was shown for the S. mutans sloABC promoter (42).

FIG 4
  • Open in new tab
  • Download powerpoint
FIG 4

The S. mutans mntH gene belongs to the SloR regulon. (A) qRT-PCR analysis indicates that expression levels of mntH and sloA were upregulated in a ΔsloR strain compared to the parent strain UA159. Data represent means ± and standard deviations of results from 3 independent experiments. Student's t test was used to compare differences in gene expression between UA159 and ΔsloR strains. (B) Regulation of the S. mutans mntH gene by SloR is direct. EMSA was performed with a [γ-32P]ATP end-labeled mntH probe and purified SloR. Reaction mixtures were resolved on 12% nondenaturing polyacrylamide gels and exposed to X-ray film for 24 h at –80°C. The addition of cold competitor DNA (1:1) or 3 mM EDTA in the SloR-mntH reaction mixture abrogated the band shift, whereas addition of 300 nM SloR resulted in a supershift. (C) Sequence of the mntH regulatory region. The predicted −35 and −10 regions are indicated with a solid underline, and the putative ribosome binding site (RBS) is indicated with a dashed underline. The translational start codon is shown in bold italics, while the predicted SloR recognition element (SRE) containing two hexamers is indicated in bold roman characters.

Manganese is critical for S. mutans tolerance of clinically relevant conditions.To examine the importance of Mn in the oxidative stress tolerance of S. mutans, we first grew cells in the presence of a subinhibitory concentration of H2O2. Under the conditions tested, growth of the parent strain or of the ΔsloC strain or ΔmntH strain was not affected; however, the growth rates and yields of the ΔsloC ΔmntH double mutant strain were markedly reduced (Fig. 5A). Importantly, this growth defect was rescued by Mn supplementation (Fig. 5B). In parallel, we tested this same panel of strains in a qualitative competition assay against the net H2O2-producing oral commensals Streptococcus gordonii and Streptococcus sanguinis. While the antagonizing peroxigenic strain inhibited growth of all S. mutans strains, the growth inhibition of the ΔsloC ΔmntH strain was much more pronounced (Fig. 5C). The inhibitory effect of the peroxigenic streptococci was abolished by the addition of catalase.

FIG 5
  • Open in new tab
  • Download powerpoint
FIG 5

Manganese transport contributes to H2O2 tolerance. (A and B) Growth of S. mutans UA159, ΔsloC, ΔmntH, and ΔsloC ΔmntH strains in the presence of 0.2 mM H2O2 in (A) plain BHI agar or (B) BHI agar supplemented with 10 μM Mn. (C) A peroxigenic strain (S. gordonii DL-1 or S. sanguinis) SK150 was spotted at the center of a BHI agar plate (supplemented with 2 μM Mn) and grown for 24 h (37°C, 5% CO2). S. mutans cultures were then spotted proximal to the peroxigenic strain and grown for an additional 24 h. The center spot of each grouping shown here is the H2O2-producing strain, while the S. mutans strains are labeled in the figure (ΔΔ corresponds to the ΔsloC ΔmntH double mutant). As a control, duplicate spotting was performed in which H2O2 produced by the peroxigenic strains was neutralized by overlaying the inoculum spot with a catalase solution prior to spotting of S. mutans. The images shown are representative of results from three independent experiments.

The ability to withstand acid stress is a major virulence attribute of S. mutans that sets it apart as a cariogenic organism compared to the less aciduric commensal streptococci. Recently, the S. agalactiae MntH was shown to play a crucial role in low-pH survival (39). To probe the significance of Mn in acid stress, cultures of parent and mutant strains were grown in FMC medium adjusted to pH 7.0 (control) or pH 5.5 (acid stress) and containing the concentration of Mn indicated in the original recipe (130 μM Mn) or containing the minimal concentration of Mn (3 μM Mn) that sustained optimal growth of the ΔsloC ΔmntH strain in FMC medium (Fig. 6A). In medium adjusted to pH 7.0, all strains grew well and reached the same final growth yield under conditions of a high or low Mn concentration (data not shown). In medium adjusted to pH 5.5, all strains reached similar final growth yields under the high-Mn condition (130 μM Mn) (Fig. 6B). However, all strains showed reduced final growth yields in the low-Mn medium adjusted to pH 5.5 (compared to high-Mn medium). Moreover, the final growth of the ΔmntH and ΔsloC ΔmntH strains was further impaired in the low-Mn medium adjusted to pH 5.5 (Fig. 6B). Collectively, these results reveal that a minimal threshold of intracellular Mn is a determining factor for the oxidative and acid stress tolerance of S. mutans.

FIG 6
  • Open in new tab
  • Download powerpoint
FIG 6

Manganese transport contributes to acid stress tolerance in S. mutans. (A) Growth curves showing the minimal concentration of Mn that fully supports growth of the ΔsloC ΔmntH strain. The graphs represent averages and standard deviations of results from three independent cultures. (B) Growth of S. mutans UA159, ΔsloC, ΔmntH, or ΔsloC ΔmntH in FMC medium adjusted to pH 5.5 containing ∼130 μM Mn (High Mn; solid bars), or 3 μM Mn (low Mn; striped bars). Bars represent means and standard deviations of the final OD600 values for five independent experiments. The horizontal line represents the mean final OD600 for UA159 grown in FMC medium containing low Mn. Student's t test was used to compare the final values determined for the mutant strains to those determined for UA159 grown in the same medium. *, P < 0.05.

Manganese promotes sucrose-dependent biofilm formation.Next, we investigated the ability of the Mn transport mutant strains to adhere and form biofilms on saliva-coated microtiter plate wells using BHI medium supplemented with 2% sucrose. In the early stage of biofilm development (4 h of incubation), all mutant strains showed a significant defect in biofilm formation, with the ΔsloC ΔmntH strain showing the most pronounced defect (∼85% reduction) (Fig. 7A). Supplementation of the growth media with Mn partially restored the early-stage biofilm defect of the double mutant strain (Fig. 7A). After the mature biofilm was formed (24 h of incubation), only the ΔsloC ΔmntH double mutant continued to show a statistically significant defect in biofilm formation (∼25% reduction); this phenotype was fully restored by Mn supplementation (Fig. 7B). Despite the slow-growth phenotype of the ΔsloC ΔmntH double mutant in BHI broth (Fig. 2B), no differences in growth (based on optical density at 600 nm [OD600] and CFU counts) were observed among strains at the two time points shown in Fig. 7 (data not shown). Collectively, these results support previous observations indicating that the ability to maintain intracellular Mn homeostasis is important for sucrose-dependent biofilm formation.

FIG 7
  • Open in new tab
  • Download powerpoint
FIG 7

Manganese acquisition is important for sucrose-dependent biofilm formation of S. mutans UA159. Cultures were grown in BHI broth containing 2% sucrose with or without supplementation with 10 μM Mn for 4 or 24 h in saliva-coated microtiter wells. The graph shows averages and standard deviations of results from three independent experiments performed in quadruplicate. **, P ≤ 0.05; ***, P ≤ 0.01; ****, P ≤ 0.005.

Growth and survival of the ΔsloC ΔmntH strain was impaired in human saliva ex vivo.As a resident of the human oral cavity, S. mutans is bathed in saliva; therefore, the ability to proliferate and survive in this biological fluid is an important aspect of its lifestyle. Here, we tested the ability of parent and mutant strains to grow and survive in pooled human saliva supplemented with 10 mM glucose to promote a more robust level of cell growth. Metal quantifications revealed that our batch of pooled saliva had relatively high Fe (4.51 ± 0.08 μM) and low Zn (0.4 ± 0.02 μM) levels whereas the level of Mn was below the detection limit (Table 2). The parent and single mutant strains grew well in saliva, showing a peak increase in CFU of nearly 2 logs of growth within the initial 18 h, followed by a noticeable loss of cell viability after 48 h (Fig. 8A). On the other hand, the ΔsloC ΔmntH strain grew poorly within the initial few hours and rapidly lost viability, eventually yielding no viable cells by 48 h. Supplementation of the saliva-glucose media with 10 μM Mn allowed all strains (including ΔsloC ΔmntH) to reach maximal growth yields faster and to maintain viability comparable to that of the parent strain during the initial 24 h (Fig. 8B).

FIG 8
  • Open in new tab
  • Download powerpoint
FIG 8

Manganese transport is critical for S. mutans growth and survival in human saliva. Strains (UA159, ΔsloC, ΔmntH, or ΔsloC ΔmntH) were grown in plain BHI agar to an OD600 of ∼0.3, washed in PBS, and diluted 1:20 in (A) pooled saliva containing 10 mM glucose or (B) pooled saliva supplemented with 10 mM glucose and 10 μM Mn. The graphs show averages and standard deviations of results from four independent experiments.

SloABC and MntH are required for calprotectin tolerance.The bioavailability of metals in body fluids is largely dependent on the presence and activity of metal-sequestering proteins such as transferrin, lactoferrin, and calprotectin. In the case of Mn, calprotectin is the major host protein responsible for sequestering Mn (as well as zinc) during infection (3, 6). Recent work revealed that the metal binding properties of calprotectin are more expansive than initially believed, importantly bringing to light the ability of calprotectin to bind to iron in vivo (7, 43). Normally found in circulating blood and tissues at low levels, calprotectin accumulates to concentrations of up to 1 mg ml−1 in response to inflammation and infection, thereby playing a central role in host-activated nutritional immunity. The apparent ability of calprotectin to scavenge reactive oxygen species adds a further dimension to the relationships among this protein, the host, and the pathogen during infection (6, 44). Here, we tested the ability of S. mutans parent and Mn transport mutants to grow in the presence of subinhibitory concentrations of purified calprotectin (Fig. 9). We found that 150 μg ml−1 calprotectin significantly delayed growth of the ΔsloC mutant and nearly abolished growth of the ΔsloC ΔmntH double mutant (Fig. 9B). At 200 μg ml−1 of calprotectin, growth of both ΔsloC and ΔsloC ΔmntH strains was fully inhibited (Fig. 9C). In contrast, the parent and ΔmntH strains grown in the presence of calprotectin showed an extended lag phase compared to cells grown in calprotectin-free media; that result did not impact final growth yields compared to cells grown under control conditions (Fig. 9A to C). Finally, the growth-inhibitory effect of calprotectin at 200 μg ml−1 on the ΔsloC and ΔsloC ΔmntH strains was fully overcome by supplementation with 20 μM Mn (Fig. 9D).

FIG 9
  • Open in new tab
  • Download powerpoint
FIG 9

SloABC and MntH are required for S. mutans tolerance of calprotectin. Data represent growth of UA159 and its derivatives in the presence of purified calprotectin (CP). Overnight cultures were diluted 1:20 into BHI agar, grown to early log phase (OD600 = 0.25), and then diluted 1:50 in CP medium containing (A) no CP, (B) 150 μg ml−1 CP, (C) 200 μg ml−1 CP, or (D) 200 μg ml−1 CP plus 20 μM Mn. The graphs show averages and standard deviations of results from three independent cultures.

DISCUSSION

In this study, we showed that Mn is an essential micronutrient for S. mutans and that the ability to maintain Mn homeostasis is important for the expression of virulence factors associated with oral and nonoral infections. Global transcriptional profiling of S. mutans UA159 grown under Mn-depleted conditions led to the identification of a previously uncharacterized Mn transporter, here named MntH, belonging to the Nramp family of transporters. By studying the physiology of the ΔsloC, ΔmntH, and ΔsloC ΔmntH strains, we provided unequivocal evidence that SloABC and MntH are the primary Mn transporters in S. mutans and that simultaneous inactivation of sloC and mntH impaired the fitness of S. mutans under Mn-restricted conditions. However, the ΔsloC ΔmntH double mutant strain retained the ability to grow under Mn-rich conditions. While the genome of S. mutans does not encode additional transporters with homology to other known manganese transporters, the promiscuous import of metals via noncognate metal transporters and even as part of a complex with another (nonmetal) substrate has been well documented. While evidence showing a major reduction in the levels of intracellular Mn pools and the most severe phenotypes was restricted to the ΔsloC ΔmntH double mutant, deletion of sloC alone significantly impaired growth of S. mutans in the chemically defined media lacking both Mn and Fe as well as in the presence of calprotectin. This finding is in agreement with previous observations made with S. aureus showing that the staphylococcal SloABC homologue, named MntABC, was more important than MntH during infection and that loss of mntABC alone resulted in a virulence defect (45). The apparent more prominent role of SloABC than of MntH seen under these specific conditions is likely due to its dual function in Fe and Mn uptake.

Although Nramp-type proteins have been shown to transport different types of trace metal ions such as Fe, Mn, and Zn (46), recent studies performed with S. agalactiae and E. faecalis revealed that the closest homologs of the S. mutans MntH are primarily involved in Mn transport (32, 39). This appears to be the case for S. mutans MntH, as the intracellular levels of Fe or Zn were minimally affected by mntH inactivation (Fig. 3). Future studies should include analysis of intracellular metal content from cells grown in media deprived of selected metals to ascertain the specificity of transporters for various metals. Note that while Nramp transporters are commonly found in bacteria, members of this family are absent in some major pathogenic streptococci such as S. pyogenes and S. pneumoniae. On the other hand, all streptococcal genomes encode one copy of an ABC-type Mn transporter homologous to SloABC, though the genetic organizations of the subunits may differ (47). Both of these transporters are known to have multiple membrane-spanning segments. Predictive analysis using TMpred (https://embnet.vital-it.ch/software/TMPRED_form.html) software indicates that the SloB membrane-spanning subunit contains 7 transmembrane helices whereas MntH displays 10 membrane-spanning domains.

In S. mutans, inactivation of sloABC resulted in attenuated virulence in a rat model of infectious endocarditis (38) whereas inactivation of the lone Mn transporter in S. pneumoniae abrogated virulence in systemic, respiratory tract, and otitis media infections (33). In E. faecalis OG1RF, which encodes one ABC-type (EfaCBA) and two Nramp-type (MntH1 and MntH2) Mn transporters, inactivation of efaCBA and mntH2 virtually abolished the virulence of E. faecalis in mammalian models (32). In the future, it will be useful to test the virulence potential of the S. mutans ΔmntH and ΔsloC ΔmntH strains in an animal model of infective endocarditis, as we suspect that simultaneous disruption of mntH and the sloABC operon would abrogate the ability of S. mutans to cause systemic infections, yielding a much more robust phenotype than the single ΔsloABC mutant strain displayed (38).

After sloABC, mntH, and the sloR repressor, the next group of overexpressed genes in cells starved for Mn belonged to the CRISPR2 system (∼5-fold average gene upregulation), which is thought to provide sequence-based immunity against “invasion” by mobile genetic elements (48). CRISPRs are often associated with a set of cas genes that encode proteins that mediate the defense process. In S. mutans UA159, deletion of the cas genes associated with CRISPR2 increased cell sensitivity to heat shock without affecting cell sensitivity to the virulent phage M102 (49). A second CRISPR system present in S. mutans UA159, named CRISPR1, was shown to mediate tolerance toward multiple stresses, including membrane, DNA, and oxidative and heat stress (49). While the mechanism remains to be determined, it seems that CRISPR systems are intimately associated with S. mutans stress responses. Among the genes downregulated under the Mn-depleted condition, 38 genes belong to genomic islands (GI) TnSmu1 (25 genes), a 23-kb region that lies adjacent to a cluster of tRNA genes, and TnSmu2 (13 genes), the largest genomic island found in UA159 (50). While not much is known about the biological roles of these GI in S. mutans, TnSmu2 is responsible for the biosynthesis of a pigment important for oxidative stress tolerance (51). It is also noteworthy that genes belonging to CRISPR systems and to TnSmu1 and TnSmu2 are also differentially expressed in strains lacking the serine protease clpP, the transcriptional regulator covR, and cidB from the Cid/Lrg holin/antiholin system (52–54). Even though ClpP, CovR, and Cid/Lrg modulate diverse biological processes, they seem to share a role in stress tolerance and adaptation. For these reasons, studies to investigate the possible association of these mobile genetic elements with metal homeostasis should be considered in the near future.

SloR was previously shown to repress transcription of the sloABC operon in a Mn-dependent fashion by binding to conserved palindromes that define a so-called SloR recognition element (SRE) in the sloABC promoter region (35, 36). As a result, growth of S. mutans in Mn-rich media resulted in decreased sloABC transcription (36, 38, 55). Previously, a genome-wide characterization of S. mutans UA159 identified a putative SRE in the mntH promoter region (29). Here, our results confirm that SloR contributes to the regulation of mntH, though the results of both RNAseq and qRT-PCR analyses indicate that SloR repression of the sloABC operon is tighter than it is for the mntH gene. Such robust sloABC repression by SloR can be explained by our previous characterization of cooperative, homodimeric binding between SloR and each of three hexameric repeats that overlap the sloABC promoter (42). Whether SloR binding at the mntH locus is cooperative and whether the SloR binding sites overlap the mntH promoter remain to be determined. While the EMSA results described here support the idea of the presence of two or more SloR binding sites upstream of the mntH gene, how this might translate into greater promoter accessibility to RNA polymerase, and thus into more-relaxed mntH transcription, warrants further investigation.

The immune protein calprotectin has been shown to play a critical role in hampering the progress of infections associated with pathogens occupying a range of host niches, including Staphylococcus aureus, Helicobacter pylori, Candida albicans, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterococcus faecalis (32, 56–60). Though earlier reports suggested that calprotectin was incapable of binding Fe in the host environment, new evidence has emerged indicating that calprotectin can starve bacteria for iron in selected media as well as under certain in vivo conditions (3, 32, 43, 56–62). Importantly, enzymatic function of the S. mutans superoxide dismutase (SOD) is heavily dependent on Mn for protection from oxidative stresses. Though the enzyme is cambialistic (capable of using either Mn or Fe), studies have shown that the Mn-bound SOD is much more active than Fe-bound SOD (63). Evidence has suggested that restriction of the Mn-dependent SOD by metal sequestration is an important aspect of the contribution of calprotectin to nutritional immunity for S. aureus pathogenesis and that the staphylococcal MntH and MntABC manganese transporters are critical for infection (45, 58).

Previous epidemiological studies have associated high availability of trace metal in the oral cavity with a higher caries incidence in predetermined populations (12–16). In particular, Mn appears to play a prominent role in host-pathogen interactions by serving as a cofactor for bacterial enzymes involved in general metabolism, DNA replication, and oxidative stress tolerance (28). The association of Mn levels with the physiology and cariogenicity of oral streptococci was first examined in the late 1960s and became the subject of more-intensive investigations from the mid-1980s until the early 1990s. Collectively, studies have shown that Mn (i) is an essential cofactor for both cariogenic and noncariogenic streptococci, (ii) plays a major role in the growth of S. mutans at elevated oxygen levels by serving as a cofactor of the superoxide dismutase enzyme, (iii) modulates dextran-mediated aggregation in different species of oral streptococci, and (iv) stimulates carbohydrate metabolism and IPS accumulation in S. mutans (17, 18, 21, 22, 24, 26, 64). Most notably, when added to drinking water, Mn resulted in a significant increase in the total number of carious lesions as well as caries severity in germfree WAGG rats (21). Despite the important advances enabled by those studies, most were conducted prior to or in the early days of the genomic era, when the currently available tools for molecular genetic manipulations and comparative genomics were under development. Here, taking advantage of the contemporary tools available, we confirmed some of those initial discoveries and further expanded our understanding of how Mn influences the pathophysiology of S. mutans. In this report, we confirmed or showed for the first time that some of the major cariogenic traits of S. mutans, such as acid and oxidative stress tolerance, survival in saliva, and sucrose-dependent biofilm formation, are in fact dependent on the intracellular levels of Mn. Further, we have demonstrated that manganese transporters are critical to the ability of S. mutans to tolerate the host immune protein calprotectin, which pathogens encounter in the oral cavity and, particularly, in the bloodstream. These results suggest that strategies to deprive S. mutans of Mn hold great promise in our efforts to combat this important pathogen.

MATERIALS AND METHODS

Bacterial strains and growth conditions.The bacterial strains used in this study are listed in Table 3. S. mutans UA159 and its derivatives were routinely grown in BHI agar supplemented with 75 μM MnSO4 at 37°C under anaerobic conditions. For physiologic analyses, bacterial inocula were prepared from overnight cultures grown in BHI medium supplemented with 7 μM MnSO4 (BHI+Mn), subcultured 1:20 in plain BHI medium (without Mn supplementation), and grown to the early logarithmic phase (OD600 = 0.25) at 37°C in a 5% CO2 atmosphere. To assess the ability of S. mutans strains to grow in BHI medium or the chemically defined FMC medium (65), cultures prepared as indicated above were diluted 1:50 into the appropriate medium in a microtiter plate with an overlay of sterile mineral oil to minimize the deleterious effects of oxygen metabolism. Growth was monitored using a BioScreen C growth reader (Oy Growth Curves) at 37°C. Growth in the presence of calprotectin requires the use of 38% bacterial medium and 62% CP buffer (20 mM Tris [pH 7.5], 100 mM NaCl, 3 mM CaCl2, 5 mM β-mercaptoethanol). To promote the growth of S. mutans in the CP medium, 3×-concentrated BHI medium was used in combination with the CP buffer. For RNA-Seq analysis, three replicate cultures of UA159 were grown overnight in plain BHI medium as described above and then subcultured 1:20 in complete FMC medium (containing 130 μM Mn) as a control or in Mn-depleted FMC medium in which Mn was omitted from the recipe. Cultures were grown to an OD600 of 0.4, harvested by centrifugation, and the bacterial pellets were resuspended in 1 ml RNA Protect bacterial reagent (Qiagen). Following another centrifugation cycle, the supernatants were discarded and the pellets stored at –80°C until use.

View this table:
  • View inline
  • View popup
  • Download powerpoint
TABLE 3

Bacterial strains used in this study

Construction of mutant and complemented strains.S. mutans strains lacking the sloC gene or the mntH gene or both were constructed using a PCR ligation mutagenesis approach (66). Briefly, PCR fragments flanking the region to be deleted were ligated to an antibiotic resistance cassette (erythromycin for the ΔsloC strain and spectinomycin for the ΔmntH strain) and the ligation mixture was used to transform S. mutans UA159 according to an established protocol (66). The double mutant strain was obtained by amplifying the ΔmntH region and using the resulting DNA amplicon to transform the ΔsloC single mutant strain. Mutant strains were isolated on BHI plates supplemented with 75 μM Mn and the appropriate antibiotic(s). Gene deletions were confirmed by sequencing amplicons containing the antibiotic cassette insertion site and flanking region. The double mutant strain was complemented by cloning the full-length sloC or mntH gene into the S. mutans integration vector pMC340B (67) to yield plasmid pMC340B-sloC or pMC340B-mntH. The plasmids were propagated in Escherichia coli DH10B and used to transform the S. mutans ΔsloC ΔmntH strain for integration at the mtl locus. All primers used in this study are listed in Table 4.

View this table:
  • View inline
  • View popup
  • Download powerpoint
TABLE 4

Primers used in this study

RNA analysis.Total RNA was isolated from homogenized S. mutans cell lysates by acid-phenol-chloroform extractions as previously described (68). The RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were resuspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 min at 37°C. Then, 100 μg of RNA per sample was purified using an RNeasy kit (Qiagen) including a second on-column DNase digestion according to the manufacturer’s instructions. Sample quality and quantity were assessed on an Agilent 2100 Bioanalyzer at the University of Florida Interdisciplinary Center for Biotechnology Research (UF-ICBR). RNA (5 μg per sample) was subjected to two rounds of mRNA enrichment using a MICROBExpress bacterial mRNA purification kit (Thermo Fisher). cDNA libraries with unique barcodes were generated from 100 ng enriched mRNA using an NEB Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs). The individual cDNA libraries were assessed for quality and quantity by Qubit. The cDNA libraries were then diluted to 10 nM each, and equimolar amounts were pooled together. The pooled libraries were subjected to RNA deep sequencing (RNA-Seq) at the UF-ICBR using an Illumina NextSeq 500 platform. Read mapping was performed on a Galaxy server hosted by the University of Florida Research Computer using Map with Bowtie for Illumina and the S. mutans UA159 genome (GenBank accession no. NC_004350.2) as a reference. The reads per open reading frame were tabulated with htseq-count. Final comparisons between the control and Mn-depleted conditions were performed with Degust (http://degust.erc.monash.edu/), with a false-discovery rate (FDR) of 0.05 and a 2-fold change cutoff. Quantifications of mntH and sloA mRNA were obtained by quantitative real-time PCR (qRT-PCR) using gene-specific primers (Table 4) on triplicate samples of the S. mutans UA159 and GMS584 (ΔsloR) strains grown to mid-logarithmic phase (OD600 of 0.5) according to established protocols (42). Student's t test was applied to the analysis of the qRT-PCR results.

ICP-OES analysis.The total metal content within bacterial cells was determined using ICP-OES performed at the University of Florida Institute of Food and Agricultural Sciences (UF-IFAS) Analytical Services Laboratories. Briefly, cultures (250 ml) were grown in plain BHI medium to mid-exponential phase (OD600 = 0.4), harvested by centrifugation at 4°C for 15 min at 4,000 rpm, and washed first in phosphate-buffered saline (PBS) supplemented with 0.2 mM EDTA to chelate extracellular divalent cations followed by a wash in PBS alone. The bacterial pellets were resuspended in 2 ml 35% HNO3 and digested at 90°C for 1 h in a high-density polyethylene scintillation vial. The digested bacteria were diluted 1:10 in reagent-grade H2O prior to ICP-OES metal analysis. The metal composition was quantified using a 5300DV ICP atomic emission spectrometer (PerkinElmer), and concentrations were determined by comparisons to a standard curve. Metal concentrations were then normalized to total protein content as determined by the bicinchoninic acid (BCA) assay (Pierce).

Growth antagonism assay.The ability of S. gordonii or S. sanguinis to inhibit the growth of S. mutans via H2O2 production was assessed as described previously (69, 70). Briefly, 8 μl of an overnight culture of S. gordonii DL-1 or S. sanguinis SK150 was spotted in the center of a BHI+Mn agar plate and incubated at 37°C and 5% CO2. After 24 h incubation, 8 μl of S. mutans overnight cultures grown in BHI+Mn were spotted near the peroxigenic strain and were similarly allowed to incubate overnight before monitoring for proximal growth defects was performed. To confirm that growth inhibition was due to H2O2 production, a control condition was included in which 8 μl of 1 mg ml−1 catalase solution was spotted on top of the peroxigenic strain spot prior to spotting the S. mutans culture.

Growth and survival in human saliva.To test the ability of the S. mutans strains to proliferate and survive in saliva, pooled human saliva was subjected to filter sterilization using a 0.2-μm-pore-size membrane and heat inactivation at 65°C for 30 min. Cultures of S. mutans grown in BHI medium to an OD600 of 0.25 as described above were then diluted 1:20 into filtered saliva supplemented either with 10 mM glucose or with 10 mM glucose and 10 μM MnSO4 prior to incubation at 37°C in a 5% CO2 atmosphere. Immediately upon dilution in saliva and at selected time intervals, 10-fold serial dilutions were prepared in sterile PBS and plated onto BHI+Mn agar for viable plate counting. Saliva samples were collected after obtaining written consent per the study approval from the University of Florida Internal Review Board (Protocol 201600877).

Biofilm assay.The ability of S. mutans strains to form biofilms on saliva-coated wells of polystyrene microtiter plates was assessed by growing cells in BHI medium supplemented with 1% sucrose with or without 10 μM of Mn. The wells of the plates were first coated for 30 min with 100 μl of sterile clarified and pooled human saliva. Next, strains grown in BHI+Mn to an OD600 of 0.5 were diluted 1:100 in BHI medium containing 1% sucrose and were added to the wells of the microtiter plate. Plates were incubated at 37°C in a 5% CO2 atmosphere for 4 and 24 h. After incubation, plates were washed twice with water to remove planktonic and loosely bound bacteria, and adherent cells were stained with 0.1% crystal violet for 15 min. The bound dye was eluted with 33% acetic acid solution, and biofilm formation was then quantified by measuring the optical density of the solution at 575 nm.

Electrophoretic mobility shift assays.EMSAs were performed according to established protocols (42). Briefly, primers were designed to amplify the promoter regions of the S. mutans mntH gene (Table 4). The resulting amplicons were subjected to end labeling with [γ-32P]dATP (Perkin-Elmer) in the presence of T4 polynucleotide kinase (New England BioLabs), after which they were centrifuged through a TE Select-D G-25 spin column (Roche Applied Science) to remove unincorporated [32P]dATP. Binding reactions were prepared using 16-μl reaction mixtures containing 1 μl (∼13.25 ng) of end-labeled amplicon, purified native SloR protein at concentrations ranging from 0 to 400 nM, and 3.2 μl of 5× binding buffer (42 mM NaH2PO4, 58 mM Na2HPO4, 250 mM NaCl, 25 mM MgCl2, 50 mg ml−1 bovine serum albumin, 1 mg sonicated salmon sperm DNA, 50% glycerol, 37.5 M MnCl2). Samples were loaded onto 12% nondenaturing polyacrylamide gels and resolved at 300 V for 1.5 h. Gels were exposed to Kodak BioMax film for 24 h at 80°C in the presence of an intensifying screen prior to autoradiography.

Data availability.Gene expression data have been deposited in the NCBI Gene Expression Omnibus (GEO) database (https://www.ncbi.nlm.nih.gov/geo) under GEO Series accession number GSE139093.

ACKNOWLEDGMENTS

Purified calprotectin was generously provided by Eric Skaar and Walter Chazin at Vanderbilt University.

This study was supported by NIH-NIDCR R01 DE019783 and NIH-NIAID R21 AI137446 to J.A.L. and NIH-NIDCR R01 DE014711 to G.A.S.

FOOTNOTES

    • Received October 21, 2019.
    • Accepted December 6, 2019.
  • Copyright © 2020 Kajfasz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

REFERENCES

  1. 1.↵
    1. Andreini C,
    2. Bertini I,
    3. Cavallaro G,
    4. Holliday GL,
    5. Thornton JM
    . 2008. Metal ions in biological catalysis: from enzyme databases to general principles. J Biol Inorg Chem 13:1205–1218. doi:10.1007/s00775-008-0404-5.
    OpenUrlCrossRefPubMedWeb of Science
  2. 2.↵
    1. Corbin BD,
    2. Seeley EH,
    3. Raab A,
    4. Feldmann J,
    5. Miller MR,
    6. Torres VJ,
    7. Anderson KL,
    8. Dattilo BM,
    9. Dunman PM,
    10. Gerads R,
    11. Caprioli RM,
    12. Nacken W,
    13. Chazin WJ,
    14. Skaar EP
    . 2008. Metal chelation and inhibition of bacterial growth in tissue abscesses. Science 319:962–965. doi:10.1126/science.1152449.
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Damo SM,
    2. Kehl-Fie TE,
    3. Sugitani N,
    4. Holt ME,
    5. Rathi S,
    6. Murphy WJ,
    7. Zhang Y,
    8. Betz C,
    9. Hench L,
    10. Fritz G,
    11. Skaar EP,
    12. Chazin WJ
    . 2013. Molecular basis for manganese sequestration by calprotectin and roles in the innate immune response to invading bacterial pathogens. Proc Natl Acad Sci U S A 110:3841–3846. doi:10.1073/pnas.1220341110.
    OpenUrlAbstract/FREE Full Text
  4. 4.↵
    1. Hood MI,
    2. Skaar EP
    . 2012. Nutritional immunity: transition metals at the pathogen-host interface. Nat Rev Microbiol 10:525–537. doi:10.1038/nrmicro2836.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Palmer LD,
    2. Skaar EP
    . 2016. Transition metals and virulence in bacteria. Annu Rev Genet 50:67–91. doi:10.1146/annurev-genet-120215-035146.
    OpenUrlCrossRefPubMed
  6. 6.↵
    1. Zackular JP,
    2. Chazin WJ,
    3. Skaar EP
    . 2015. Nutritional immunity: S100 proteins at the host-pathogen interface. J Biol Chem 290:18991–18998. doi:10.1074/jbc.R115.645085.
    OpenUrlAbstract/FREE Full Text
  7. 7.↵
    1. Zygiel EM,
    2. Nolan EM
    . 2019. Exploring iron withholding by the innate immune protein human calprotectin. Acc Chem Res 52:2301–2308. doi:10.1021/acs.accounts.9b00250.
    OpenUrlCrossRef
  8. 8.↵
    1. Abranches J,
    2. Zeng L,
    3. Kajfasz JK,
    4. Palmer SR,
    5. Chakraborty B,
    6. Wen ZT,
    7. Richards VP,
    8. Brady LJ,
    9. Lemos JA
    . 2018. Biology of oral streptococci. Microbiol Spectr 6(5). doi:10.1128/microbiolspec.GPP3-0042-2018.
    OpenUrlCrossRef
  9. 9.↵
    1. Selwitz RH,
    2. Ismail AI,
    3. Pitts NB
    . 2007. Dental caries. Lancet 369:51–59. doi:10.1016/S0140-6736(07)60031-2.
    OpenUrlCrossRefPubMedWeb of Science
  10. 10.↵
    1. Lemos JA,
    2. Burne RA
    . 2008. A model of efficiency: stress tolerance by Streptococcus mutans. Microbiology 154:3247–3255. doi:10.1099/mic.0.2008/023770-0.
    OpenUrlCrossRefPubMedWeb of Science
  11. 11.↵
    1. Pant S,
    2. Patel NJ,
    3. Deshmukh A,
    4. Golwala H,
    5. Patel N,
    6. Badheka A,
    7. Hirsch GA,
    8. Mehta JL
    . 2015. Trends in infective endocarditis incidence, microbiology, and valve replacement in the United States from 2000 to 2011. J Am Coll Cardiol 65:2070–2076. doi:10.1016/j.jacc.2015.03.518.
    OpenUrlFREE Full Text
  12. 12.↵
    1. Curzon ME,
    2. Crocker DC
    . 1978. Relationships of trace elements in human tooth enamel to dental caries. Arch Oral Biol 23:647–653. doi:10.1016/0003-9969(78)90189-9.
    OpenUrlCrossRefPubMed
  13. 13.↵
    1. Duggal MS,
    2. Chawla HS,
    3. Curzon ME
    . 1991. A study of the relationship between trace elements in saliva and dental caries in children. Arch Oral Biol 36:881–884. doi:10.1016/0003-9969(91)90118-e.
    OpenUrlCrossRefPubMedWeb of Science
  14. 14.↵
    1. Glass RL,
    2. Rothman KJ,
    3. Espinal F,
    4. Velez H,
    5. Smith NJ
    . 1973. The prevalence of human dental caries and water-borne trace metals. Arch Oral Biol 18:1099–1104. doi:10.1016/0003-9969(73)90083-6.
    OpenUrlCrossRefPubMedWeb of Science
  15. 15.↵
    1. Green I
    . 1970. Copper and manganese in saliva of children. J Dent Res 49:776–782. doi:10.1177/00220345700490041201.
    OpenUrlCrossRefPubMed
  16. 16.↵
    1. Tsanidou E,
    2. Nena E,
    3. Rossos A,
    4. Lendengolts Z,
    5. Nikolaidis C,
    6. Tselebonis A,
    7. Constantinidis TC
    . 2015. Caries prevalence and manganese and iron levels of drinking water in school children living in a rural/semi-urban region of north-eastern Greece. Environ Health Prev Med 20:404–409. doi:10.1007/s12199-015-0482-2.
    OpenUrlCrossRef
  17. 17.↵
    1. Aranha H,
    2. Strachan RC,
    3. Arceneaux JE,
    4. Byers BR
    . 1982. Effect of trace metals on growth of Streptococcus mutans in a Teflon chemostat. Infect Immun 35:456–460.
    OpenUrlAbstract/FREE Full Text
  18. 18.↵
    1. Arirachakaran P,
    2. Luengpailin S,
    3. Banas JA,
    4. Mazurkiewicz JE,
    5. Benjavongkulchai E
    . 2007. Effects of manganese on Streptococcus mutans planktonic and biofilm growth. Caries Res 41:497–502. doi:10.1159/000110882.
    OpenUrlCrossRefPubMed
  19. 19.↵
    1. Bauer PD,
    2. Trapp C,
    3. Drake D,
    4. Taylor KG,
    5. Doyle RJ
    . 1993. Acquisition of manganous ions by mutans group streptococci. J Bacteriol 175:819–825. doi:10.1128/jb.175.3.819-825.1993.
    OpenUrlAbstract/FREE Full Text
  20. 20.↵
    1. Beighton D
    . 1980. Manganese antagonizes the inhibitory effect of fluoride on the glucose metabolism of Streptococcus mutans. Microbios 28:149–156.
    OpenUrlPubMed
  21. 21.↵
    1. Beighton D
    . 1982. The influence of manganese on carbohydrate metabolism and caries induction by Streptococcus mutans strain Ingbritt. Caries Res 16:189–192. doi:10.1159/000260596.
    OpenUrlCrossRefPubMed
  22. 22.↵
    1. Bowen WH
    . 1968. The trace element requirements of cariogenic and non-cariogenic streptococci. Arch Oral Biol 13:713–714. doi:10.1016/0003-9969(68)90151-9.
    OpenUrlCrossRefPubMed
  23. 23.↵
    1. Bowen WH
    . 1971. The effects of calcium, magnesium and manganese on dextran production by a cariogenic streptococcus. Arch Oral Biol 16:115–119. doi:10.1016/0003-9969(71)90142-7.
    OpenUrlCrossRefPubMed
  24. 24.↵
    1. Lü-Lü,
    2. Singh JS,
    3. Galperin MY,
    4. Drake D,
    5. Taylor KG,
    6. Doyle RJ
    . 1992. Chelating agents inhibit activity and prevent expression of streptococcal glucan-binding lectins. Infect Immun 60:3807–3813.
    OpenUrlAbstract/FREE Full Text
  25. 25.↵
    1. Strachan RC,
    2. Aranha H,
    3. Lodge JS,
    4. Arceneaux JE,
    5. Byers BR
    . 1982. Teflon chemostat for studies of trace metal metabolism in Streptococcus mutans and other bacteria. Appl Environ Microbiol 43:257–260.
    OpenUrlAbstract/FREE Full Text
  26. 26.↵
    1. Drake D,
    2. Taylor KG,
    3. Doyle RJ
    . 1988. Expression of the glucan-binding lectin of Streptococcus cricetus requires manganous ion. Infect Immun 56:2205–2207.
    OpenUrlAbstract/FREE Full Text
  27. 27.↵
    1. Bowen WH,
    2. Koo H
    . 2011. Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res 45:69–86. doi:10.1159/000324598.
    OpenUrlCrossRefPubMed
  28. 28.↵
    1. Juttukonda LJ,
    2. Skaar EP
    . 2015. Manganese homeostasis and utilization in pathogenic bacteria. Mol Microbiol 97:216–228. doi:10.1111/mmi.13034.
    OpenUrlCrossRefPubMed
  29. 29.↵
    1. Turner AG,
    2. Djoko KY,
    3. Ong CY,
    4. Barnett TC,
    5. Walker MJ,
    6. McEwan AG
    . 2019. Group A Streptococcus co-ordinates manganese import and iron efflux in response to hydrogen peroxide stress. Biochem J 476:595–611. doi:10.1042/BCJ20180902.
    OpenUrlAbstract/FREE Full Text
  30. 30.↵
    1. Chicharro JL,
    2. Serrano V,
    3. Ureña R,
    4. Gutierrez AM,
    5. Carvajal A,
    6. Fernández-Hernando P,
    7. Lucía A
    . 1999. Trace elements and electrolytes in human resting mixed saliva after exercise. Br J Sports Med 33:204–207. doi:10.1136/bjsm.33.3.204.
    OpenUrlAbstract
  31. 31.↵
    1. Scheuhammer AM,
    2. Cherian MG
    . 1985. Binding of manganese in human and rat plasma. Biochim Biophys Acta 840:163–169. doi:10.1016/0304-4165(85)90115-1.
    OpenUrlCrossRefPubMed
  32. 32.↵
    1. Colomer-Winter C,
    2. Flores-Mireles AL,
    3. Baker SP,
    4. Frank KL,
    5. Lynch AJL,
    6. Hultgren SJ,
    7. Kitten T,
    8. Lemos JA
    . 2018. Manganese acquisition is essential for virulence of Enterococcus faecalis. PLoS Pathog 14:e1007102. doi:10.1371/journal.ppat.1007102.
    OpenUrlCrossRef
  33. 33.↵
    1. Marra A,
    2. Lawson S,
    3. Asundi JS,
    4. Brigham D,
    5. Hromockyj AE
    . 2002. In vivo characterization of the psa genes from Streptococcus pneumoniae in multiple models of infection. Microbiology 148:1483–1491. doi:10.1099/00221287-148-5-1483.
    OpenUrlCrossRefPubMedWeb of Science
  34. 34.↵
    1. Kitten T,
    2. Munro CL,
    3. Michalek SM,
    4. Macrina FL
    . 2000. Genetic characterization of a Streptococcus mutans LraI family operon and role in virulence. Infect Immun 68:4441–4451. doi:10.1128/iai.68.8.4441-4451.2000.
    OpenUrlAbstract/FREE Full Text
  35. 35.↵
    1. Crepps SC,
    2. Fields EE,
    3. Galan D,
    4. Corbett JP,
    5. Von Hasseln ER,
    6. Spatafora GA
    . 2016. The SloR metalloregulator is involved in the Streptococcus mutans oxidative stress response. Mol Oral Microbiol 31:526–539. doi:10.1111/omi.12147.
    OpenUrlCrossRef
  36. 36.↵
    1. O'Rourke KP,
    2. Shaw JD,
    3. Pesesky MW,
    4. Cook BT,
    5. Roberts SM,
    6. Bond JP,
    7. Spatafora GA
    . 2010. Genome-wide characterization of the SloR metalloregulome in Streptococcus mutans. J Bacteriol 192:1433–1443. doi:10.1128/JB.01161-09.
    OpenUrlAbstract/FREE Full Text
  37. 37.↵
    1. Rolerson E,
    2. Swick A,
    3. Newlon L,
    4. Palmer C,
    5. Pan Y,
    6. Keeshan B,
    7. Spatafora G
    . 2006. The SloR/Dlg metalloregulator modulates Streptococcus mutans virulence gene expression. J Bacteriol 188:5033–5044. doi:10.1128/JB.00155-06.
    OpenUrlAbstract/FREE Full Text
  38. 38.↵
    1. Paik S,
    2. Brown A,
    3. Munro CL,
    4. Cornelissen CN,
    5. Kitten T
    . 2003. The sloABCR operon of Streptococcus mutans encodes an Mn and Fe transport system required for endocarditis virulence and its Mn-dependent repressor. J Bacteriol 185:5967–5975. doi:10.1128/jb.185.20.5967-5975.2003.
    OpenUrlAbstract/FREE Full Text
  39. 39.↵
    1. Shabayek S,
    2. Bauer R,
    3. Mauerer S,
    4. Mizaikoff B,
    5. Spellerberg B
    . 2016. A streptococcal NRAMP homologue is crucial for the survival of Streptococcus agalactiae under low pH conditions. Mol Microbiol 100:589–606. doi:10.1111/mmi.13335.
    OpenUrlCrossRef
  40. 40.↵
    1. Ganguly T,
    2. Kajfasz JK,
    3. Miller JH,
    4. Rabinowitz E,
    5. Galvão LCC,
    6. Rosalen PL,
    7. Abranches J,
    8. Lemos JA,
    9. Ganguly T,
    10. Kajfasz JK,
    11. Miller JH,
    12. Rabinowitz E,
    13. Galvão LCC,
    14. Rosalen PL,
    15. Abranches J,
    16. Lemos JA
    . 2018. Disruption of a novel iron transport system reverses oxidative stress phenotypes of a dpr mutant strain of Streptococcus mutans. J Bacteriol 200:e00062-18. doi:10.1128/JB.00062-18.
    OpenUrlAbstract/FREE Full Text
  41. 41.↵
    1. Spatafora G,
    2. Corbett J,
    3. Cornacchione L,
    4. Daly W,
    5. Galan D,
    6. Wysota M,
    7. Tivnan P,
    8. Collins J,
    9. Nye D,
    10. Levitz T,
    11. Breyer WA,
    12. Glasfeld A
    . 2015. Interactions of the metalloregulatory protein SloR from Streptococcus mutans with its metal ion effectors and DNA binding site. J Bacteriol 197:3601–3615. doi:10.1128/JB.00612-15.
    OpenUrlAbstract/FREE Full Text
  42. 42.↵
    1. Monette P,
    2. Brach R,
    3. Cowan A,
    4. Winters R,
    5. Weisman J,
    6. Seybert F,
    7. Goguen K,
    8. Chen J,
    9. Glasfeld A,
    10. Spatafora G,
    11. Monette P,
    12. Brach R,
    13. Cowan A,
    14. Winters R,
    15. Weisman J,
    16. Seybert F,
    17. Goguen K,
    18. Chen J,
    19. Glasfeld A,
    20. Spatafora G
    . 2018. Autoregulation of the Streptococcus mutans SloR metalloregulator is constitutive and driven by an independent promoter. J Bacteriol 200:e00214-18. doi:10.1128/JB.00214-18.
    OpenUrlAbstract/FREE Full Text
  43. 43.↵
    1. Zygiel EM,
    2. Nolan EM
    . 2018. Transition metal sequestration by the host-defense protein calprotectin. Annu Rev Biochem 87:621–643. doi:10.1146/annurev-biochem-062917-012312.
    OpenUrlCrossRefPubMed
  44. 44.↵
    1. Goyette J,
    2. Geczy CL
    . 2011. Inflammation-associated S100 proteins: new mechanisms that regulate function. Amino Acids 41:821–842. doi:10.1007/s00726-010-0528-0.
    OpenUrlCrossRefPubMedWeb of Science
  45. 45.↵
    1. Radin JN,
    2. Zhu J,
    3. Brazel EB,
    4. McDevitt CA,
    5. Kehl-Fie TE
    . 19 December 2018, posting date. Synergy between nutritional immunity and independent host defenses contributes to the importance of the MntABC manganese transporter during Staphylococcus aureus infection. Infect Immun doi:10.1128/IAI.00642-18.
    OpenUrlCrossRef
  46. 46.↵
    1. Nevo Y,
    2. Nelson N
    . 2006. The NRAMP family of metal-ion transporters. Biochim Biophys Acta 1763:609–620. doi:10.1016/j.bbamcr.2006.05.007.
    OpenUrlCrossRefPubMedWeb of Science
  47. 47.↵
    1. Crump KE,
    2. Bainbridge B,
    3. Brusko S,
    4. Turner LS,
    5. Ge X,
    6. Stone V,
    7. Xu P,
    8. Kitten T
    . 2014. The relationship of the lipoprotein SsaB, manganese and superoxide dismutase in Streptococcus sanguinis virulence for endocarditis. Mol Microbiol 92:1243–1259. doi:10.1111/mmi.12625.
    OpenUrlCrossRefPubMed
  48. 48.↵
    1. Barrangou R,
    2. Fremaux C,
    3. Deveau H,
    4. Richards M,
    5. Boyaval P,
    6. Moineau S,
    7. Romero DA,
    8. Horvath P
    . 2007. CRISPR provides acquired resistance against viruses in prokaryotes. Science 315:1709–1712. doi:10.1126/science.1138140.
    OpenUrlAbstract/FREE Full Text
  49. 49.↵
    1. Serbanescu MA,
    2. Cordova M,
    3. Krastel K,
    4. Flick R,
    5. Beloglazova N,
    6. Latos A,
    7. Yakunin AF,
    8. Senadheera DB,
    9. Cvitkovitch DG
    . 2015. Role of the Streptococcus mutans CRISPR-Cas systems in immunity and cell physiology. J Bacteriol 197:749–761. doi:10.1128/JB.02333-14.
    OpenUrlAbstract/FREE Full Text
  50. 50.↵
    1. Ajdic D,
    2. McShan WM,
    3. McLaughlin RE,
    4. Savic G,
    5. Chang J,
    6. Carson MB,
    7. Primeaux C,
    8. Tian R,
    9. Kenton S,
    10. Jia H,
    11. Lin S,
    12. Qian Y,
    13. Li S,
    14. Zhu H,
    15. Najar F,
    16. Lai H,
    17. White J,
    18. Roe BA,
    19. Ferretti JJ
    . 2002. Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc Natl Acad Sci U S A 99:14434–14439. doi:10.1073/pnas.172501299.
    OpenUrlAbstract/FREE Full Text
  51. 51.↵
    1. Wu C,
    2. Cichewicz R,
    3. Li Y,
    4. Liu J,
    5. Roe B,
    6. Ferretti J,
    7. Merritt J,
    8. Qi F
    . 2010. Genomic island TnSmu2 of Streptococcus mutans harbors a nonribosomal peptide synthetase-polyketide synthase gene cluster responsible for the biosynthesis of pigments involved in oxygen and H2O2 tolerance. Appl Environ Microbiol 76:5815–5826. doi:10.1128/AEM.03079-09.
    OpenUrlAbstract/FREE Full Text
  52. 52.↵
    1. Ahn SJ,
    2. Rice KC
    . 2016. Understanding the Streptococcus mutans Cid/Lrg System through CidB Function. Appl Environ Microbiol 82:6189–6203. doi:10.1128/AEM.01499-16.
    OpenUrlAbstract/FREE Full Text
  53. 53.↵
    1. Chattoraj P,
    2. Banerjee A,
    3. Biswas S,
    4. Biswas I
    . 2010. ClpP of Streptococcus mutans differentially regulates expression of genomic islands, mutacin production, and antibiotic tolerance. J Bacteriol 192:1312–1323. doi:10.1128/JB.01350-09.
    OpenUrlAbstract/FREE Full Text
  54. 54.↵
    1. Dmitriev A,
    2. Mohapatra SS,
    3. Chong P,
    4. Neely M,
    5. Biswas S,
    6. Biswas I
    . 2011. CovR-controlled global regulation of gene expression in Streptococcus mutans. PLoS One 6:e20127. doi:10.1371/journal.pone.0020127.
    OpenUrlCrossRefPubMed
  55. 55.↵
    1. Spatafora G,
    2. Moore M,
    3. Landgren S,
    4. Stonehouse E,
    5. Michalek S
    . 2001. Expression of Streptococcus mutans fimA is iron-responsive and regulated by a DtxR homologue. Microbiology 147:1599–1610. doi:10.1099/00221287-147-6-1599.
    OpenUrlCrossRefPubMed
  56. 56.↵
    1. Gaddy JA,
    2. Radin JN,
    3. Loh JT,
    4. Piazuelo MB,
    5. Kehl-Fie TE,
    6. Delgado AG,
    7. Ilca FT,
    8. Peek RM,
    9. Cover TL,
    10. Chazin WJ,
    11. Skaar EP,
    12. Scott Algood HM
    . 2014. The host protein calprotectin modulates the Helicobacter pylori cag type IV secretion system via zinc sequestration. PLoS Pathog 10:e1004450. doi:10.1371/journal.ppat.1004450.
    OpenUrlCrossRefPubMed
  57. 57.↵
    1. Hood MI,
    2. Mortensen BL,
    3. Moore JL,
    4. Zhang Y,
    5. Kehl-Fie TE,
    6. Sugitani N,
    7. Chazin WJ,
    8. Caprioli RM,
    9. Skaar EP
    . 2012. Identification of an Acinetobacter baumannii zinc acquisition system that facilitates resistance to calprotectin-mediated zinc sequestration. PLoS Pathog 8:e1003068. doi:10.1371/journal.ppat.1003068.
    OpenUrlCrossRefPubMed
  58. 58.↵
    1. Kehl-Fie TE,
    2. Zhang Y,
    3. Moore JL,
    4. Farrand AJ,
    5. Hood MI,
    6. Rathi S,
    7. Chazin WJ,
    8. Caprioli RM,
    9. Skaar EP
    . 2013. MntABC and MntH contribute to systemic Staphylococcus aureus infection by competing with calprotectin for nutrient manganese. Infect Immun 81:3395–3405. doi:10.1128/IAI.00420-13.
    OpenUrlAbstract/FREE Full Text
  59. 59.↵
    1. Urban CF,
    2. Ermert D,
    3. Schmid M,
    4. Abu-Abed U,
    5. Goosmann C,
    6. Nacken W,
    7. Brinkmann V,
    8. Jungblut PR,
    9. Zychlinsky A
    . 2009. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans. PLoS Pathog 5:e1000639. doi:10.1371/journal.ppat.1000639.
    OpenUrlCrossRefPubMed
  60. 60.↵
    1. Zygiel EM,
    2. Nelson CE,
    3. Brewer LK,
    4. Oglesby-Sherrouse AG,
    5. Nolan EM
    . 2019. The human innate immune protein calprotectin induces iron starvation responses in Pseudomonas aeruginosa. J Biol Chem 294:3549–3562. doi:10.1074/jbc.RA118.006819.
    OpenUrlAbstract/FREE Full Text
  61. 61.↵
    1. Besold AN,
    2. Gilston BA,
    3. Radin JN,
    4. Ramsoomair C,
    5. Culbertson EM,
    6. Li CX,
    7. Cormack BP,
    8. Chazin WJ,
    9. Kehl-Fie TE,
    10. Culotta VC
    . 22 January 2018, posting date. Role of calprotectin in withholding zinc and copper from Candida albicans. Infect Immun doi:10.1128/IAI.00779-17.
    OpenUrlCrossRef
  62. 62.↵
    1. Wang J,
    2. Lonergan ZR,
    3. Gonzalez-Gutierrez G,
    4. Nairn BL,
    5. Maxwell CN,
    6. Zhang Y,
    7. Andreini C,
    8. Karty JA,
    9. Chazin WJ,
    10. Trinidad JC,
    11. Skaar EP,
    12. Giedroc DP
    . 2019. Multi-metal restriction by calprotectin impacts de novo flavin biosynthesis in Acinetobacter baumannii. Cell Chem Biol 26:745–755.e7. doi:10.1016/j.chembiol.2019.02.011.
    OpenUrlCrossRef
  63. 63.↵
    1. De Vendittis A,
    2. Amato M,
    3. Mickniewicz A,
    4. Parlato G,
    5. De Angelis A,
    6. Castellano I,
    7. Rullo R,
    8. Riccitiello F,
    9. Rengo S,
    10. Masullo M,
    11. De Vendittis E
    . 2010. Regulation of the properties of superoxide dismutase from the dental pathogenic microorganism Streptococcus mutans by iron- and manganese-bound co-factor. Mol Biosyst 6:1973–1982. doi:10.1039/c003557b.
    OpenUrlCrossRefPubMed
  64. 64.↵
    1. Martin ME,
    2. Strachan RC,
    3. Aranha H,
    4. Evans SL,
    5. Salin ML,
    6. Welch B,
    7. Arceneaux JE,
    8. Byers BR
    . 1984. Oxygen toxicity in Streptococcus mutans: manganese, iron, and superoxide dismutase. J Bacteriol 159:745–749.
    OpenUrlAbstract/FREE Full Text
  65. 65.↵
    1. Terleckyj B,
    2. Willett NP,
    3. Shockman GD
    . 1975. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun 11:649–655.
    OpenUrlAbstract/FREE Full Text
  66. 66.↵
    1. Lau PC,
    2. Sung CK,
    3. Lee JH,
    4. Morrison DA,
    5. Cvitkovitch DG
    . 2002. PCR ligation mutagenesis in transformable streptococci: application and efficiency. J Microbiol Methods 49:193–205. doi:10.1016/s0167-7012(01)00369-4.
    OpenUrlCrossRefPubMedWeb of Science
  67. 67.↵
    1. Chen PM,
    2. Chen YY,
    3. Yu SL,
    4. Sher S,
    5. Lai CH,
    6. Chia JS
    . 2010. Role of GlnR in acid-mediated repression of genes encoding proteins involved in glutamine and glutamate metabolism in Streptococcus mutans. Appl Environ Microbiol 76:2478–2486. doi:10.1128/AEM.02622-09.
    OpenUrlAbstract/FREE Full Text
  68. 68.↵
    1. Abranches J,
    2. Candella MM,
    3. Wen ZT,
    4. Baker HV,
    5. Burne RA
    . 2006. Different roles of EIIABMan and EIIGlc in regulation of energy metabolism, biofilm development, and competence in Streptococcus mutans. J Bacteriol 188:3748–3756. doi:10.1128/JB.00169-06.
    OpenUrlAbstract/FREE Full Text
  69. 69.↵
    1. Kajfasz JK,
    2. Rivera-Ramos I,
    3. Scott-Anne K,
    4. Gregoire S,
    5. Abranches J,
    6. Lemos JA
    . 2015. Transcription of oxidative stress genes is directly activated by SpxA1 and, to a lesser extent, by SpxA2 in Streptococcus mutans. J Bacteriol 197:2160–2170. doi:10.1128/JB.00118-15.
    OpenUrlAbstract/FREE Full Text
  70. 70.↵
    1. Kreth J,
    2. Zhang Y,
    3. Herzberg MC
    . 2008. Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans. J Bacteriol 190:4632–4640. doi:10.1128/JB.00276-08.
    OpenUrlAbstract/FREE Full Text
View Abstract
PreviousNext
Back to top
Download PDF
Citation Tools
Manganese Uptake, Mediated by SloABC and MntH, Is Essential for the Fitness of Streptococcus mutans
Jessica K. Kajfasz, Callahan Katrak, Tridib Ganguly, Jonathan Vargas, Logan Wright, Zachary T. Peters, Grace A. Spatafora, Jacqueline Abranches, José A. Lemos
mSphere Jan 2020, 5 (1) e00764-19; DOI: 10.1128/mSphere.00764-19

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print
Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this mSphere article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Manganese Uptake, Mediated by SloABC and MntH, Is Essential for the Fitness of Streptococcus mutans
(Your Name) has forwarded a page to you from mSphere
(Your Name) thought you would be interested in this article in mSphere.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Manganese Uptake, Mediated by SloABC and MntH, Is Essential for the Fitness of Streptococcus mutans
Jessica K. Kajfasz, Callahan Katrak, Tridib Ganguly, Jonathan Vargas, Logan Wright, Zachary T. Peters, Grace A. Spatafora, Jacqueline Abranches, José A. Lemos
mSphere Jan 2020, 5 (1) e00764-19; DOI: 10.1128/mSphere.00764-19
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

S. mutans
manganese
metal transport
stress response
dental caries
biofilm
Streptococcus mutans

Related Articles

Cited By...

About

  • About mSphere
  • Board of Editors
  • Policies
  • For Reviewers
  • For the Media
  • Embargo Policy
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Author Warranty
  • Types of Articles
  • Getting Started
  • Ethics
  • Contact Us

Follow #mSphereJ

@ASMicrobiology

       

 

Website feedback

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Online ISSN: 2379-5042