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Research Article | Synthetic Biology

Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis

Vinh G. Tran, Mingfeng Cao, Zia Fatma, Xiaofei Song, Huimin Zhao
Aaron P. Mitchell, Editor
Vinh G. Tran
aDepartment of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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Mingfeng Cao
aDepartment of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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Zia Fatma
aDepartment of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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Xiaofei Song
aDepartment of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
bDepartment of Microbiology, Nankai University, Tianjin, China
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Huimin Zhao
aDepartment of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
cDepartment of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
dDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
eDepartment of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
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Aaron P. Mitchell
Carnegie Mellon University
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DOI: 10.1128/mSphere.00345-19
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Article Information

DOI 
https://doi.org/10.1128/mSphere.00345-19
PubMed 
31243078

Published By 
American Society for Microbiology Journals
History 
  • Received May 14, 2019
  • Accepted June 11, 2019
  • Published online June 26, 2019.

Copyright & Usage 
Copyright © 2019 Tran et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Aaron P. Mitchell, Editor, Carnegie Mellon University

Author Information

  1. Vinh G. Trana,
  2. Mingfeng Caoa,
  3. Zia Fatmaa,
  4. Xiaofei Songa,b and
  5. Huimin Zhaoa,c,d,e
  1. aDepartment of Chemical and Biomolecular Engineering, Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  2. bDepartment of Microbiology, Nankai University, Tianjin, China
  3. cDepartment of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  4. dDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  5. eDepartment of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  • Address correspondence to Huimin Zhao, zhao5{at}illinois.edu.
  • V.G.T. and M.C. contributed equally to this work.

  • Citation Tran VG, Cao M, Fatma Z, Song X, Zhao H. 2019. Development of a CRISPR/Cas9-based tool for gene deletion in Issatchenkia orientalis. mSphere 4:e00345-19. https://doi.org/10.1128/mSphere.00345-19.

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Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
Vinh G. Tran, Mingfeng Cao, Zia Fatma, Xiaofei Song, Huimin Zhao
mSphere Jun 2019, 4 (3) e00345-19; DOI: 10.1128/mSphere.00345-19

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Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis
Vinh G. Tran, Mingfeng Cao, Zia Fatma, Xiaofei Song, Huimin Zhao
mSphere Jun 2019, 4 (3) e00345-19; DOI: 10.1128/mSphere.00345-19
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KEYWORDS

CRISPR/Cas9
Issatchenkia orientalis
genome editing
metabolic engineering
synthetic biology

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