Article Figures & Data
Figures
- FIG 1
Phylogenetic tree of Mycobacterium uberis and selected mycobacterial species. The tree was created in MEGA7 from concatenated amino acid sequences (3,696 positions) of 10 proteins (DnaN, RplI, GrpE, MetG, RplY, PheT, FtsQ, HolA, MiaA, and FtsY) (18) and inferred by using the maximum likelihood method based on the JTT matrix-based model. The tree is drawn to scale, with branch lengths measured at the number of substitutions per site. Bootstrap support values, estimated from 500 replicates, are given below each branch. Mycobacteroides abscessus (previously Mycobacterium abscessus) was used as the outgroup.
Tables
- TABLE 1
Genomic features of M. uberis and close relatives M. leprae, M. lepromatosis, and M. haemophilum
Feature Value M. uberis M. leprae M. lepromatosis M. haemophilum Genome size (bp) 3,122,721 3,268,212 3,206,741 4,235,765 No. of protein-coding genes 1,759 1,609 1,477 3,749 No. of pseudogenes 1,081 1,309 1,334 225 % GC content (genome) 57.49 57.80 57.89 63.95 % GC content (CDS)a 59.58 60.11 60.16 64.35 % GC content (pseudogenes) 55.60 56.45 56.59 64.84 % GC content (intergenic) 53.08 54.17 54.61 60.37 ↵a CDS, coding DNA sequence.
- TABLE 2
Samples used for the PCR detection of M. uberisa
Sample ID Animal Diagnosis Tissue Herd Animal ID Reference
or source13Z000257 Goat NT Mammary gland A goat 1 5 13Z002358 Goat NT Inguinal lymph node A goat 1 5 14Z002623 Goat NT Unspecified tissue A goat 2 5 14Z002624 Goat NT Mammary gland A goat 2 5 15Z001519 Goat NT Mammary gland B goat 3 This study 15Z001520 Goat NT Mammary gland B goat 4 This study NA Cow NT Mammary gland C cow 1 4 NA Cow NT Mammary gland C cow 2 4 NA Cow NT Mammary gland D cow 4 This study NA Bull TS Scrotum E taurus 1 This study NA Bull TS Scrotum E taurus 2 This study NA Bull TS Scrotum E taurus 3 This study ↵a ID, identifier; NT, nodular thelitis; TS, tuberculoid scrotitis; NA, not available.
TEXT S1
Supplemental Materials and Methods. Download Text S1, PDF file, 0.1 MB.
Copyright © 2018 Benjak et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
DATA SET S1
Mycobacterium uberis Jura genome annotation. Download Data Set S1, XLSX file, 1.8 MB.
Copyright © 2018 Benjak et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
FIG S1
Genetic organization of the ESX-1 locus and the functionally associated espACD operon in M. uberis and comparison with M. tuberculosis, M. haemophilum, M. leprae, and M. lepromatosis. Colored arrows represent the various ESX genes encoding proteins belonging to different protein families (after Simeone et al.) (19). Empty arrows represent pseudogenes. Slashes separate distant genomic regions. Download FIG S1, EPS file, 0.1 MB.
Copyright © 2018 Benjak et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
FIG S2
Maximum likelihood phylogenetic tree of Mycobacterium uberis and selected mycobacterial species. The tree was created in MEGA7 based on the Tamura-Nei model from 372 nucleotide positions of the groEL2 (hsp65) gene. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Bootstrap support values, estimated from 500 replicates, are given below each branch. Download FIG S2, EPS file, 0.1 MB.
Copyright © 2018 Benjak et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
