Article Figures & Data
Figures
- FIG 1
Phred analysis of Illumina and MinION reads for the F. nucleatum subsp. nucleatum ATCC 25586 genome. (A) Q scores for Illumina reads. (B) Q scores (Phred quality) for MinION reads 20 to 80 kb in length. (C) Q scores for MinION reads up to 20 kb in length.
- FIG 2
Statistics and mapping of F. necrophorum funduliforme 1_1_36S MinION long reads. After complete genome assembly, MinION reads were mapped to the F. necrophorum funduliforme 1_1_36S genome using Geneious version 9.1.4 software.
- FIG 3
Genome assembly and annotation of eight Fusobacterium genomes from seven species. Short-read-only and complete genome assembly representations were created using Bandage (21).
- FIG 4
Analysis of Fusobacterium nucleatum ATCC 23726 genomes compared to previous builds. (A) Alignment of the complete F. nucleatum subsp. nucleatum ATCC 23726 genome with the 67-contig draft assembly (GenBank accession number ADVK01000000). (B) Confirmation of a 452-kb genomic inversion in the previous F. nucleatum subsp. nucleatum ATCC 25586 genome assembly (GenBank accession number GCA_000007325.1).
Tables
- TABLE 1
Data deposited at NCBI for all sequenced Fusobacterium strains
Species Strain GenBank genome
accession no.BioProject
accession no.BioSample
accession no.SRAa Illumina
accession no.SRA MinION
accession no.F. nucleatum 23726 GCA_003019785.1 PRJNA433545 SAMN08501025 SRX3740879 SRX3740878 F. nucleatum 25586 GCA_003019295.1 PRJNA433545 SAMN08706662 SRX3786193 SRX3786192 F. varium 27725 GCA_003019655.1 PRJNA433545 SAMN08501142 SRX3740889 SRX3740888 F. ulcerans 49185 GCA_003019675.1 PRJNA433545 SAMN08501141 SRX3740885 SRX3740884 F. mortiferum 9817 GCA_003019315.1 PRJNA433545 SAMN08501148 SRX3740887 SRX3740886 F. gonidiaformans 25563 GCA_003019695.1 PRJNA433545 SAMN08501140 SRX3740881 SRX3740880 F. periodonticum 2_1_31 GCA_003019755.1 PRJNA433545 SAMN08501101 SRX3740877 SRX3740876 F. necrophorum 1_1_36S GCA_003019715.1 PRJNA433545 SAMN08501105 SRX3740883 SRX3740882 ↵a SRA, sequence read archive at NCBI.
TABLE S1
Statistics for short-read Illumina sequencing. Download TABLE S1, PDF file, 0.1 MB.
Copyright © 2018 Todd et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S2
Experimental details of MinION sequencing. Download TABLE S2, PDF file, 0.1 MB.
Copyright © 2018 Todd et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
TABLE S3
Results of MinION sequencing. Download TABLE S3, PDF file, 0.1 MB.
Copyright © 2018 Todd et al.
This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.
