Article Figures & Data
Figures
- FIG 1
(A) Ventral dissection of adult female squid showing the nidamental glands (NG), accessory nidamental gland (ANG), and light organ (LO). (B) Map of Oahu, HI, showing locations of two squid populations, Kaneohe Bay (21°25′44.0″N, 157°47′32.4″W) and Maunalua Bay (21°16′51.42″N, 157°43′33.07″W).
- FIG 2
Bray-Curtis beta diversity analysis of Kaneohe Bay (KB) ANG and JC bacterial communities. (A) The overall community composition of KB and Maunalua Bay (MB) ANGs and JCs overlapped, but the KB samples had lower dispersion and clustered apart from the MB samples. (B) ANG and JC bacterial community compositions were not distinct in Kaneohe Bay. (C) KB ANG and JC samples clustered by associated female. Ellipses represent 95% confidence intervals. Results of ANOSIM are presented in parentheses in each plot.
- FIG 3
Alpha diversity analysis of Kaneohe Bay ANG and JC bacterial communities. Two-way ANOVA (see Table S1 in the supplemental material) revealed no effect of population or tissue type on bacterial community richness/evenness (A), phylogenetic diversity (B), or richness informed by the number of rare taxa present (C). Thick bars indicate means; thin bars indicate standard deviations.
- FIG 4
(A) Kaneohe Bay (KB) ANG and JC bacterial communities resembled those observed in Maunalua Bay (MB) at the class level. (B) KB animals had significantly more Gammaproteobacteria in their ANGs than MB hosts (t36 = 5.129, P < 0.0001). (C) JCs from KB animals had significantly more Gammaproteobacteria than MB JCs (t53 = 4.73, P = 0.0001) and also fewer Flavobacteriia (t53 = 3.138, P = 0.01). Taxa are presented at the class level (Verrucomicrobiae and Opitutae are both classes within the Verrucomicrobia phylum); the scatter plot is presented on a log scale to show variation for taxa present at lower average abundances. Thick bars represent means; thin bars represent standard deviations; asterisks represent significant differences between populations (B and C). Error bars that would have extended below 0.1% sequences/sample have been omitted from the graph (B and C). The “other” component included taxa present in more than one sample and at less than 0.3% average abundance.
- FIG 5
A higher abundance of Gammaproteobacteria in the Kaneohe Bay (KB) ANG and JC bacterial communities was due to a shift in BD2-13, a genus from the Alteromonadaceae family. BD2-13 (t38 = 5.22, P = 0.0001) was significantly more abundant in KB ANGs (A) and in KB JCs (t53 = 5.612, P = 0.00001) (B). One Alphaproteobacteria taxon (Leisingera: t53 = 3.085, P = 0.04) was also significantly higher in KB JCs (B), while two others were significantly lower in KB JCs (Rhizobiales: t53 = 3.107, P = 0.04; Methylocystaceae: t53 = 3.639, P = 0.009). Taxa are presented at the finest level obtained (c, class; o, order; f, family; g, genus); the scatter plot is presented on a log scale to show variation for taxa present at lower average abundances. Thick bars represent means; thin bars represent standard deviations; asterisks represent significant differences between populations. Error bars that would have extended below 0.01% sequences/sample have been omitted from the graph (A and B). The “other” component included taxa present in more than one sample and at less than 0.3% average abundance.
Supplemental Material
TABLE S1
Two-way ANOVA of alpha diversity analysis of Kaneohe Bay ANGs and JCs (Fig. 3) found no effect of population or sample type on bacterial community richness/evenness (A), phylogenetic diversity (B), or richness informed by the number of rare taxa present (C). Thick bars indicate means; thin bars indicate standard deviations. Download TABLE S1, TIF file, 0.2 MB.
Copyright © 2018 Kerwin and Nyholm.
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