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Research Article | Host-Microbe Biology

Intracellular Trafficking and Persistence of Acinetobacter baumannii Requires Transcription Factor EB

Raquel Parra-Millán, David Guerrero-Gómez, Rafael Ayerbe-Algaba, Maria Eugenia Pachón-Ibáñez, Antonio Miranda-Vizuete, Jerónimo Pachón, Younes Smani
Sarah E. F. D'Orazio, Editor
Raquel Parra-Millán
aClinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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David Guerrero-Gómez
bRedox Homeostasis Group, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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Rafael Ayerbe-Algaba
aClinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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Maria Eugenia Pachón-Ibáñez
aClinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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Antonio Miranda-Vizuete
bRedox Homeostasis Group, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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Jerónimo Pachón
aClinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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Younes Smani
aClinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville, IBiS, University Hospital Virgen del Rocío/CSIC/University of Seville, Seville, Spain
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Sarah E. F. D'Orazio
University of Kentucky
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DOI: 10.1128/mSphere.00106-18
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ABSTRACT

Acinetobacter baumannii is a significant human pathogen associated with hospital-acquired infections. While adhesion, an initial and important step in A. baumannii infection, is well characterized, the intracellular trafficking of this pathogen inside host cells remains poorly studied. Here, we demonstrate that transcription factor EB (TFEB) is activated after A. baumannii infection of human lung epithelial cells (A549). We also show that TFEB is required for the invasion and persistence inside A549 cells. Consequently, lysosomal biogenesis and autophagy activation were observed after TFEB activation which could increase the death of A549 cells. In addition, using the Caenorhabditis elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required. These results identify TFEB as a conserved key factor in the pathogenesis of A. baumannii.

IMPORTANCE Adhesion is an initial and important step in Acinetobacter baumannii infections. However, the mechanism of entrance and persistence inside host cells is unclear and remains to be understood. In this study, we report that, in addition to its known role in host defense against Gram-positive bacterial infection, TFEB also plays an important role in the intracellular trafficking of A. baumannii in host cells. TFEB was activated shortly after A. baumannii infection and is required for its persistence within host cells. Additionally, using the C. elegans infection model by A. baumannii, the TFEB orthologue HLH-30 was required for survival of the nematode to infection, although nuclear translocation of HLH-30 was not required.

  • Copyright © 2018 Parra-Millán et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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Intracellular Trafficking and Persistence of Acinetobacter baumannii Requires Transcription Factor EB
Raquel Parra-Millán, David Guerrero-Gómez, Rafael Ayerbe-Algaba, Maria Eugenia Pachón-Ibáñez, Antonio Miranda-Vizuete, Jerónimo Pachón, Younes Smani
mSphere Mar 2018, 3 (2) e00106-18; DOI: 10.1128/mSphere.00106-18

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Intracellular Trafficking and Persistence of Acinetobacter baumannii Requires Transcription Factor EB
Raquel Parra-Millán, David Guerrero-Gómez, Rafael Ayerbe-Algaba, Maria Eugenia Pachón-Ibáñez, Antonio Miranda-Vizuete, Jerónimo Pachón, Younes Smani
mSphere Mar 2018, 3 (2) e00106-18; DOI: 10.1128/mSphere.00106-18
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KEYWORDS

Acinetobacter baumannii
Caenorhabditis elegans
HLH-30
TFEB
bacterial invasion

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