Research Article | Molecular Biology and Physiology

Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination

Sanjay Vashee, Timothy B. Stockwell, Nina Alperovich, Evgeniya A. Denisova, Daniel G. Gibson, Kyle C. Cady, Kristofer Miller, Krishna Kannan, Daniel Malouli, Lindsey B. Crawford, Alexander A. Voorhies, Eric Bruening, Patrizia Caposio, Klaus Früh
Michael J. Imperiale, Editor
DOI: 10.1128/mSphereDirect.00331-17

Article Information

DOI 
https://doi.org/10.1128/mSphereDirect.00331-17
PubMed 
28989973
Published By 
American Society for Microbiology Journals
History 
  • Received July 25, 2017
  • Accepted July 29, 2017
  • Published online October 4, 2017.
Copyright & Usage 
Copyright © 2017 Vashee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Michael J. Imperiale, Editor, University of Michigan—Ann Arbor
  • Eain Murphy, Solicited external reviewer, FORGE Life Science
  • Richard Stanton, Solicited external reviewer, Cardiff University

Author Information

  1. Sanjay Vasheea,
  2. Timothy B. Stockwella,
  3. Nina Alperovicha,
  4. Evgeniya A. Denisovaa,
  5. Daniel G. Gibsonb,
  6. Kyle C. Cadyb,
  7. Kristofer Millerb,
  8. Krishna Kannanb,
  9. Daniel Maloulid,
  10. Lindsey B. Crawfordd,
  11. Alexander A. Voorhiesa,
  12. Eric Brueningc,
  13. Patrizia Caposiod and
  14. Klaus Frühd
  1. aJ. Craig Venter Institute, Rockville, Maryland, USA
  2. bSynthetic Genomics Inc., La Jolla, California, USA
  3. cTomegavax Inc., Beaverton, Oregon, USA
  4. dVaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA
  • Address correspondence to Klaus Früh, fruehk{at}ohsu.edu.

  • Citation Vashee S, Stockwell TB, Alperovich N, Denisova EA, Gibson DG, Cady KC, Miller K, Kannan K, Malouli D, Crawford LB, Voorhies AA, Bruening E, Caposio P, Früh K. 2017. Cloning, assembly, and modification of the primary human cytomegalovirus isolate Toledo by yeast-based transformation-associated recombination. mSphere 2:e00331-17. https://doi.org/10.1128/mSphereDirect.00331-17.

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KEYWORDS

Saccharomyces cerevisiae
cloning
cytomegalovirus
genetic recombination

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