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Research Article | Molecular Biology and Physiology

Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination

Sanjay Vashee, Timothy B. Stockwell, Nina Alperovich, Evgeniya A. Denisova, Daniel G. Gibson, Kyle C. Cady, Kristofer Miller, Krishna Kannan, Daniel Malouli, Lindsey B. Crawford, Alexander A. Voorhies, Eric Bruening, Patrizia Caposio, Klaus Früh
Michael J. Imperiale, Editor
Sanjay Vashee
a J. Craig Venter Institute, Rockville, Maryland, USA
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Timothy B. Stockwell
a J. Craig Venter Institute, Rockville, Maryland, USA
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Nina Alperovich
a J. Craig Venter Institute, Rockville, Maryland, USA
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Evgeniya A. Denisova
a J. Craig Venter Institute, Rockville, Maryland, USA
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Daniel G. Gibson
b Synthetic Genomics Inc., La Jolla, California, USA
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Kyle C. Cady
b Synthetic Genomics Inc., La Jolla, California, USA
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Kristofer Miller
b Synthetic Genomics Inc., La Jolla, California, USA
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Krishna Kannan
b Synthetic Genomics Inc., La Jolla, California, USA
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Daniel Malouli
d Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA
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Lindsey B. Crawford
d Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA
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Alexander A. Voorhies
a J. Craig Venter Institute, Rockville, Maryland, USA
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Eric Bruening
c Tomegavax Inc., Beaverton, Oregon, USA
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Patrizia Caposio
d Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA
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Klaus Früh
d Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA
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Michael J. Imperiale
University of Michigan—Ann Arbor
Roles: Editor
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Eain Murphy
FORGE Life Science
Roles: Solicited external reviewer
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Richard Stanton
Cardiff University
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DOI: 10.1128/mSphereDirect.00331-17
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Article Information

DOI 
https://doi.org/10.1128/mSphereDirect.00331-17
PubMed 
28989973

Published By 
American Society for Microbiology Journals
History 
  • Received July 25, 2017
  • Accepted July 29, 2017
  • Published online October 4, 2017.

Copyright & Usage 
Copyright © 2017 Vashee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license .
Editors and / or Reviewers 
  • Michael J. Imperiale, Editor, University of Michigan—Ann Arbor
  • Eain Murphy, Solicited external reviewer, FORGE Life Science
  • Richard Stanton, Solicited external reviewer, Cardiff University

Author Information

  1. Sanjay Vasheea,
  2. Timothy B. Stockwella,
  3. Nina Alperovicha,
  4. Evgeniya A. Denisovaa,
  5. Daniel G. Gibsonb,
  6. Kyle C. Cadyb,
  7. Kristofer Millerb,
  8. Krishna Kannanb,
  9. Daniel Maloulid,
  10. Lindsey B. Crawfordd,
  11. Alexander A. Voorhiesa,
  12. Eric Brueningc,
  13. Patrizia Caposiod and
  14. Klaus Frühd
  1. a J. Craig Venter Institute, Rockville, Maryland, USA
  2. b Synthetic Genomics Inc., La Jolla, California, USA
  3. c Tomegavax Inc., Beaverton, Oregon, USA
  4. d Vaccine and Gene Therapy Institute, Oregon Health & Science University, Beaverton, Oregon, USA
  • Address correspondence to Klaus Früh, fruehk{at}ohsu.edu.
  • Citation Vashee S, Stockwell TB, Alperovich N, Denisova EA, Gibson DG, Cady KC, Miller K, Kannan K, Malouli D, Crawford LB, Voorhies AA, Bruening E, Caposio P, Früh K. 2017. Cloning, assembly, and modification of the primary human cytomegalovirus isolate Toledo by yeast-based transformation-associated recombination. mSphere 2:e00331-17. https://doi.org/10.1128/mSphereDirect.00331-17 .

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Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
Sanjay Vashee, Timothy B. Stockwell, Nina Alperovich, Evgeniya A. Denisova, Daniel G. Gibson, Kyle C. Cady, Kristofer Miller, Krishna Kannan, Daniel Malouli, Lindsey B. Crawford, Alexander A. Voorhies, Eric Bruening, Patrizia Caposio, Klaus Früh
mSphere Oct 2017, 2 (5) e00331-17; DOI: 10.1128/mSphereDirect.00331-17

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Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
Sanjay Vashee, Timothy B. Stockwell, Nina Alperovich, Evgeniya A. Denisova, Daniel G. Gibson, Kyle C. Cady, Kristofer Miller, Krishna Kannan, Daniel Malouli, Lindsey B. Crawford, Alexander A. Voorhies, Eric Bruening, Patrizia Caposio, Klaus Früh
mSphere Oct 2017, 2 (5) e00331-17; DOI: 10.1128/mSphereDirect.00331-17
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KEYWORDS

Saccharomyces cerevisiae
cloning
cytomegalovirus
genetic recombination

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