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  • Articles
    • Latest Articles
    • COVID-19 Research and News from ASM Journals
    • mSphere of Influence: Commentaries from Early Career Microbiologists
    • Archive
  • Topics
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Applied and Environmental Science

  • Open Access
    Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities
    Research Article | Applied and Environmental Science
    Impact of Ammonium on Syntrophic Organohalide-Respiring and Fermenting Microbial Communities

    Contamination with ammonium and chlorinated solvents has been reported in numerous subsurface environments, and these chemicals bring significant challenges for in situ bioremediation. Dehalococcoides mccartyi is able to reduce the chlorinated solvent trichloroethene to the nontoxic end product ethene. Fermentative bacteria are of central importance for...

    Anca G. Delgado, Devyn Fajardo-Williams, Kylie L. Kegerreis, Prathap Parameswaran, Rosa Krajmalnik-Brown
  • Open Access
    IS<em>26</em>-Mediated Formation of Transposons Carrying Antibiotic Resistance Genes
    Research Article | Applied and Environmental Science
    IS26-Mediated Formation of Transposons Carrying Antibiotic Resistance Genes

    In Gram-negative bacteria, IS26 recruits antibiotic resistance genes into the mobile gene pool by forming transposons carrying many different resistance genes. In addition to replicative transposition, IS26 was recently shown to use a novel conservative movement mechanism in which an incoming IS26 targets a preexisting one. Here, we have demonstrated how IS26-bounded class I transposons can be...

    Christopher J. Harmer, Ruth M. Hall
  • Open Access
    Culture-Independent Metagenomic Surveillance of Commercially Available Probiotics with High-Throughput Next-Generation Sequencing
    Research Article | Applied and Environmental Science
    Culture-Independent Metagenomic Surveillance of Commercially Available Probiotics with High-Throughput Next-Generation Sequencing

    The rapidly growing supplement industry operates without a formal premarket approval process. Consumers rely on product labels to be accurate and true. Those products containing live microbials report both identity and viability on most product labels. This study used next-generation sequencing technology as an analytical tool in conjunction with classic culture methods to examine the validity of the labels on supplement products...

    Jennifer N. Patro, Padmini Ramachandran, Tammy Barnaba, Mark K. Mammel, Jada L. Lewis, Christopher A. Elkins
  • Open Access
    Purification and Characterization of Botulinum Neurotoxin FA from a Genetically Modified <span class="named-content genus-species" id="named-content-1">Clostridium botulinum</span> Strain
    Research Article | Applied and Environmental Science
    Purification and Characterization of Botulinum Neurotoxin FA from a Genetically Modified Clostridium botulinum Strain

    Botulinum neurotoxins (BoNTs), produced by anaerobic bacteria, are the cause of the potentially deadly, neuroparalytic disease botulism. BoNTs have been classified into seven serotypes, serotypes A to G, based upon their selective neutralization by homologous antiserum, which is relevant for clinical and diagnostic purposes. Even though supportive care dramatically reduces the death rate of botulism, the only pharmaceutical intervention...

    Sabine Pellett, William H. Tepp, Marite Bradshaw, Suzanne R. Kalb, Janet K. Dykes, Guangyun Lin, Erin M. Nawrocki, Christina L. Pier, John R. Barr, Susan E. Maslanka, Eric A. Johnson
  • Open Access
    Antibiotic Resistance in Animal and Environmental Samples Associated with Small-Scale Poultry Farming in Northwestern Ecuador
    Research Article | Applied and Environmental Science
    Antibiotic Resistance in Animal and Environmental Samples Associated with Small-Scale Poultry Farming in Northwestern Ecuador

    In developing countries, small-scale poultry farming employing antibiotics as growth promoters is being advanced as an inexpensive source of protein and income. Here, we present the results of a large ecoepidemiological study examining patterns of antibiotic resistance (AR) in E. coli isolates from small-scale poultry production environments versus domestic...

    Nikolay P. Braykov, Joseph N. S. Eisenberg, Marissa Grossman, Lixin Zhang, Karla Vasco, William Cevallos, Diana Muñoz, Andrés Acevedo, Kara A. Moser, Carl F. Marrs, Betsy Foxman, James Trostle, Gabriel Trueba, Karen Levy
  • Open Access
    A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon <span class="named-content genus-species" id="named-content-1">Pantoea ananatis</span> BRT175 against the Social Amoeba <span class="named-content genus-species" id="named-content-2">Dictyostelium discoideum</span>
    Research Article | Applied and Environmental Science
    A Novel Glycolipid Biosurfactant Confers Grazing Resistance upon Pantoea ananatis BRT175 against the Social Amoeba Dictyostelium discoideum

    The genetic factors used for host interaction by the opportunistic human pathogen Pantoea ananatis are largely unknown. We identified two genes that are important for the production of a biosurfactant that confers grazing resistance against the social amoeba Dictyostelium discoideum. We show that...

    Derek D. N. Smith, Arvin Nickzad, Eric Déziel, John Stavrinides
  • Open Access
    Metagenomic Evidence for the Presence of Comammox <em>Nitrospira</em>-Like Bacteria in a Drinking Water System
    Observation | Applied and Environmental Science
    Metagenomic Evidence for the Presence of Comammox Nitrospira-Like Bacteria in a Drinking Water System

    Nitrification plays an important role in regulating the concentrations of inorganic nitrogen species in a range of environments, from drinking water and wastewater treatment plants to the oceans. Until recently, aerobic nitrification was considered to be a two-step process involving ammonia-oxidizing bacteria or archaea and nitrite-oxidizing bacteria. This process requires close cooperation between these two functional guilds for...

    Ameet J. Pinto, Daniel N. Marcus, Umer Zeeshan Ijaz, Quyen Melina Bautista-de lose Santos, Gregory J. Dick, Lutgarde Raskin

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