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  • Articles
    • Latest Articles
    • COVID-19 Research and News from ASM Journals
    • mSphere of Influence: Commentaries from Early Career Microbiologists
    • Archive
  • Topics
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Most read article(s)

  • Open Access
    Effectiveness of Face Masks in Preventing Airborne Transmission of SARS-CoV-2
    Observation | Clinical Science and Epidemiology
    Effectiveness of Face Masks in Preventing Airborne Transmission of SARS-CoV-2

    Airborne simulation experiments showed that cotton masks, surgical masks, and N95 masks provide some protection from the transmission of infective SARS-CoV-2 droplets/aerosols; however, medical masks (surgical masks and even N95 masks) could not completely block the transmission of virus droplets/aerosols even when sealed.

    Hiroshi Ueki, Yuri Furusawa, Kiyoko Iwatsuki-Horimoto, Masaki Imai, Hiroki Kabata, Hidekazu Nishimura, Yoshihiro Kawaoka
  • Open Access
    Molecular Evolution of Human Coronavirus 229E in Hong Kong and a Fatal COVID-19 Case Involving Coinfection with a Novel Human Coronavirus 229E Genogroup
    Research Article | Clinical Science and Epidemiology
    Molecular Evolution of Human Coronavirus 229E in Hong Kong and a Fatal COVID-19 Case Involving Coinfection with a Novel Human Coronavirus 229E Genogroup

    Since its first appearance in the 1960s, the genetic diversity and evolution of human coronavirus 229E (HCoV-229E) have been relatively understudied. In this study, we report a fatal case of COVID-19 coinfected with HCoV-229E in Hong Kong.

    Susanna K. P. Lau, David C. Lung, Emily Y. M. Wong, Kam Leng Aw-Yong, Antonio C. P. Wong, Hayes K. H. Luk, Kenneth S. M. Li, Joshua Fung, Tony T. Y. Chan, James Y. M. Tang, Longchao Zhu, Cyril C. Y. Yip, Sally C. Y. Wong, Rodney A. Lee, Owen T. Y. Tsang, Kwok-Yung Yuen, Patrick C. Y. Woo
  • Open Access
    Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing
    Research Article
    Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing

    In 16S rRNA gene sequencing, certain bacterial genera were found to be underrepresented or even missing in taxonomic profiles when using unsuitable primer combinations, outdated reference databases, or inadequate pipeline settings. Concerning the last, quality thresholds as well as bioinformatic settings (i.e., clustering approach, analysis pipeline, and specific adjustments such as truncation) are responsible for a number of observed...

    Isabel Abellan-Schneyder, Monica S. Matchado, Sandra Reitmeier, Alina Sommer, Zeno Sewald, Jan Baumbach, Markus List, Klaus Neuhaus
  • Open Access
    <span class="named-content genus-species" id="named-content-1">Aspergillus fumigatus</span> Strain-Specific Conidia Lung Persistence Causes an Allergic Broncho-Pulmonary Aspergillosis-Like Disease Phenotype
    Research Article | Host-Microbe Biology
    Aspergillus fumigatus Strain-Specific Conidia Lung Persistence Causes an Allergic Broncho-Pulmonary Aspergillosis-Like Disease Phenotype

    Allergic broncho-pulmonary aspergillosis (ABPA) patients often present with long-term colonization of Aspergillus fumigatus. Current understanding of ABPA pathogenesis has been complicated by a lack of long-term in vivo fungal persistence models.

    Jane T. Jones, Ko-Wei Liu, Xi Wang, Caitlin H. Kowalski, Brandon S. Ross, Kathleen A. M. Mills, Joshua D. Kerkaert, Tobias M. Hohl, Lotus A. Lofgren, Jason E. Stajich, Joshua J. Obar, Robert A. Cramer
  • Open Access
    Why Are Viruses Spiked?
    Perspective
    Why Are Viruses Spiked?

    Many viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and human immunodeficiency virus (HIV), have a structure consisting of spikes protruding from an underlying spherical surface. Research in biological and colloidal sciences has revealed secrets of why spikes exist on virus surfaces.

    Chongyang Shen, Scott A. Bradford
  • Open Access
    Genetic Modification of <em>Sodalis</em> Species by DNA Transduction
    Research Article | Applied and Environmental Science
    Genetic Modification of Sodalis Species by DNA Transduction

    A large number of economically important insects maintain intimate associations with maternally inherited endosymbiotic bacteria. Due to the inherent nature of these associations, insect endosymbionts cannot be usually isolated in pure culture or genetically manipulated.

    Chelsea M. Keller, Christopher G. Kendra, Roberto E. Bruna, David Craft, Mauricio H. Pontes
  • Open Access
    New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids
    Research Article | Host-Microbe Biology
    New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids

    Human noroviruses (HuNoVs) are highly contagious and cause acute and sporadic diarrheal illness in all age groups. In addition, chronic infections occur in immunocompromised cancer and transplant patients.

    Khalil Ettayebi, Victoria R. Tenge, Nicolas W. Cortes-Penfield, Sue E. Crawford, Frederick H. Neill, Xi-Lei Zeng, Xiaomin Yu, B. Vijayalakshmi Ayyar, Douglas Burrin, Sasirekha Ramani, Robert L. Atmar, Mary K. Estes
  • Open Access
    Genomic Background Governs Opposing Responses to Nalidixic Acid upon Megaplasmid Acquisition in <em>Pseudomonas</em>
    Research Article | Ecological and Evolutionary Science
    Genomic Background Governs Opposing Responses to Nalidixic Acid upon Megaplasmid Acquisition in Pseudomonas

    Numerous studies have demonstrated that gene transfer events (e.g., plasmid acquisition) can entail a variety of costs that arise as by-products of the incorporation of foreign DNA into established physiological and genetic systems. These costs can be ameliorated through evolutionary time by the occurrence of compensatory mutations, which stabilize the presence of a horizontally transferred region within the genome but which also may...

    David A. Baltrus, Caitlin Smith, MacKenzie Derrick, Courtney Leligdon, Zoe Rosenthal, Madison Mollico, Andrew Moore, Meara Clark
  • Open Access
    Predominance of <span class="named-content genus-species" id="named-content-1">Atopobium vaginae</span> at Midtrimester: a Potential Indicator of Preterm Birth Risk in a Nigerian Cohort
    Research Article | Host-Microbe Biology
    Predominance of Atopobium vaginae at Midtrimester: a Potential Indicator of Preterm Birth Risk in a Nigerian Cohort

    Giving birth too soon accounts for half of all newborn deaths worldwide. Clinical symptoms alone are not sufficient to identify women at risk of giving birth too early, as such a pragmatic approach to reducing the incidence of preterm birth entails developing early strategies for intervention before it materializes.

    Nkechi Martina Odogwu, Jun Chen, Chinedum Amara Onebunne, Patricio Jeraldo, Lu Yang, Stephen Johnson, Funmilola A. Ayeni, Marina R. S. Walther-Antonio, Oladapo O. Olayemi, Nicholas Chia, Akinyinka O. Omigbodun
  • Open Access
    Single-Molecule Dynamics at a Bacterial Replication Fork after Nutritional Downshift or Chemically Induced Block in Replication
    Research Article | Molecular Biology and Physiology
    Single-Molecule Dynamics at a Bacterial Replication Fork after Nutritional Downshift or Chemically Induced Block in Replication

    All cells need to adjust DNA replication, which is achieved by a well-orchestrated multiprotein complex, in response to changes in physiological and environmental conditions. For replication forks, it is extremely challenging to meet with conditions where amino acids are rapidly depleted from cells, called the stringent response, to deal with the inhibition of one of the centrally involved proteins or with DNA modifications that arrest...

    Rogelio Hernández-Tamayo, Hannah Schmitz, Peter L. Graumann

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