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  • Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
    Research Article | Molecular Biology and Physiology
    Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination

    The genomes of large DNA viruses, such as human cytomegalovirus (HCMV), are difficult to manipulate using current genetic tools, and at this time, it is not possible to obtain, molecular clones of CMV without extensive tissue culture. To overcome these limitations, we used synthetic biology tools to capture genomic fragments from viral DNA and assemble full-length genomes in yeast. Using an early passage of the HCMV isolate Toledo...

    Sanjay Vashee, Timothy B. Stockwell, Nina Alperovich, Evgeniya A. Denisova, Daniel G. Gibson, Kyle C. Cady, Kristofer Miller, Krishna Kannan, Daniel Malouli, Lindsey B. Crawford, Alexander A. Voorhies, Eric Bruening, Patrizia Caposio, Klaus Früh
    04 Oct 2017
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