TABLE 3 

Putative open reading frames and their functions in pLM58

ORF no.Start
position
Stop
position
Length
(bp)
StrandaGC content
(%)
Predicted
operonb
Function
(RAST annotationc)
1116351,635+42.81Replication initiation protein
217361900165+38.79Op1Hypothetical protein
319102665756+41.53Op1FIG00775790; hypothetical protein
43301269960335.49Op2Mobile element protein
54133337875637.04Op2Mobile element protein
6537041441,22736.51Op2Mobile element protein
757146304591+29.78Op3FIG00775444; hypothetical protein
863077224918+33.88Op3FIG00775381; hypothetical protein
976227861240+37.50Op4Pli0011 protein
1078738313441+34.01Op4Pli0010 protein
11830696461,341+36.99Op4FIG00776061; hypothetical protein
12965710028372+38.44Op4FIG00775078; hypothetical protein
131004810812765+41.05Op4Pli0008 protein
141092611456531+36.72Pli0007 protein
15118281149333635.52Pli0006 protein
161200212400399+34.83Op5Pli0005 protein
1712404139061,503+38.92Op5DNA methylase
181399114443453+36.42Op5Mobile element protein
19151521447268140.38Op6Mobile element protein
20155841518340237.56Op6Transposase and inactivated
derivative-like protein
2115776171401,365+40.44NADH peroxidase (EC 1.11.1.1)
22182551738687041.49l-Proline glycine betaine ABC transport system
permease protein ProW (TC 3.A.1.12.1)
231837618615240+32.92Op7Hypothetical protein
241861518935321+29.91Op7Pli0046 protein
251916519890726+39.94FIG00774464; hypothetical protein
262035321282930+44.95Op8Lead-, cadmium-, zinc-, and mercury-
transporting ATPase (EC 3.6.3.3)
(EC 3.6.3.5); copper-translocating
P-type ATPase (EC 3.6.3.4)
272127022235966+45.55Op8Lead-, cadmium-, zinc-, and mercury-
transporting ATPase (EC 3.6.3.3)
(EC 3.6.3.5); copper-translocating
P-type ATPase (EC 3.6.3.4)
28229722229268138.18Mobile element protein
29237852341137533.60Hypothetical protein
30241312400612631.75Hypothetical protein
312430724447141+33.33Op9Hypothetical protein
322446124829369+44.17Op9DNA topoisomerase III (EC 5.99.1.2)
332482225010189+33.33Op9Hypothetical protein
342573426156423+44.92Op10Mannose-6-phosphate isomerase
(EC 5.3.1.8)
352624026893654+39.60Op10ABC-type uncharacterized transport
system; ATPase component
362689027654765+40.13Op10YbbM seven-transmembrane-helix protein
372783928036198+41.92Mobile element protein
382836228478117+30.77Hypothetical protein
392845928578120+28.33Hypothetical protein
402857129143573+37.87Nonspecific DNA-binding protein Dps/
iron-binding ferritin-like antioxidant
protein/ferroxidase (EC 1.16.3.1)
412911229273162+30.25FIG00630535; hypothetical protein
422928829992705+43.55Op11Transcriptional regulator; Crp/Fnr family
433008530309225+42.67Op11Prolipoprotein diacylglyceryl transferase
(EC 2.4.99.-)
443045630689234+36.32Copper chaperone
4530887327911,905+41.99Copper-translocating P-type ATPase
(EC 3.6.3.4)
4635030331741,85729.35Op12Bipolar DNA helicase HerA
4736216350231,19428.14Op12FIG036446; hypothetical protein
48370633648557939.90Resolvase/integrase bin
4937324393692,046+34.55Op13Lead-, cadmium-, zinc-, and mercury-
transporting ATPase (EC 3.6.3.3)
(EC 3.6.3.5); copper-translocating P-type
ATPase (EC 3.6.3.4)
5039384405441,161+29.72Op13Multicopper oxidase
5143467405522,91636.83Op14Mobile element protein
52440254347155538.02Op14DNA invertase
534430544664360+35.28Op15Cadmium efflux system accessory protein
5444664467992,136+39.98Op15Cadmium-transporting ATPase (EC 3.6.3.3)
554686747271405+37.53Op15Multicopper oxidase
564729147836546+33.15Op15DUF1541 domain-containing protein
574798848110123+21.95Hypothetical protein
584822148820600+38.33Op16Site-specific recombinase; resolvase family
594881049037228+35.96Op16Hypothetical protein
60494354915727936.92FIG00775972; hypothetical protein
614939849634237+34.60Conserved hypothetical protein;
phage associated
6250062521762,115+40.00ATP-dependent protease ATP-binding
subunit clpL
635244353441999+35.04Op17Mobile element protein
645345753792336+36.01Op17Mobile element protein
655394654701756+32.54FIG00775080; hypothetical protein
665490655250345+41.16Op18FIG00775387; hypothetical protein
6755238565331,296+41.82Op18ImpB/MucB/SamB family protein
68568325653629729.63Op19Hypothetical protein
69577845681097530.46Op19Replication-associated protein
705814258300159+28.93Hypothetical protein
  • a The location of the open reading frame on the plus (+) or minus (−) strand is indicated.

  • b Operon prediction was performed using FGENESB (54).

  • c Annotations were performed using RAST 2.0 (52).