TABLE 3 

Resistance factors of Z. tritici field isolates with different CYP51 and MFS1 alleles relative to the CYP51WT MFS1WT genotypeb

AntifungalEC50 (ng ml−1) for
CYP51WT MFS1WT
RF fora:
CYP51TriR MFS1WTCYP51TriR MFS1MDR type ICYP51TriR MFS1MDR type IICYP51TriR MFS1MDR type III
Tolnaftate15 ± 3.5ND11.911.53.8
Terbinafine0.1 ± 0.02ND84.387.637.5
Epoxiconazole0.35 ± 0.172589591,138927
Prothiocon-azole-
desthio
0.32 ± 0.1851.973.6107144
Tebuconazole3.4 ± 0.9609546381269
Metconazole0.21 ± 0.043606555721,116
Pyrifenox0.38 ± 0.145596101,054709
  • a The CYP51TriR genotype groups together different CYP51 genotypes of field strains with cross-resistance to DMIs. The resistance factors (RFs) are expressed as the EC50 of the studied genotype/EC50 of the CYP51WT MFS1WT genotype. ND, not determined.

  • b RFs relative to tolnaftate and terbinafine are affected only by the different MFS1 alleles (no target site mutations). Therefore, these values are in boldface.