TABLE 2 

Rgg homology and presence of putative pheromone SHP in other S. pneumoniae strains

StrainNCBI accession no.Identity to Rgg0939 (%)argg accession no.bshp?SequenceDownstream of peptidec
R6 (D39 derivative)NC_003098.1Yes (100)spr0960YesMKKISKFLPILFLVMDIIIIVGGPotential 12-gene operon identical to D39; 1st gene (spr0961) encodes UDP-N-acetyl-d-mannosaminuronic
acid dehydrogenase, 9th (spr0969) encodes nikkomycin biosynthesis protein
JJANC_012466.1Yes (97)SPJ_0996YesMKKISKFLPILVLVMDIIIIPotential 3-gene operon followed by 2nd 3-gene operon; 2nd gene of 1st sequence (SPJ_0998) is annotated as lantibiotic mersacidin transporter system, 1st gene of 2nd operon (SPG_1000) is annotated as bacitracin transport ATP-binding protein (BcrA)
A45dNC_018594.1Yes (96)SPNA45_01354YesMKKISKFLPILVLVMDIIIIPotential 3-gene operon followed by 2-gene operon; 1st gene (SPNA45_01355) encodes lanthionine biosynthesis protein (LanM), 2nd (SPNA45_01356) encodes toxin secretion ABC transporter, 1st gene of 2nd operon (SPNA45_01358) encodes BcrA
Yes (86)SPNA45_00934YesMEKISKFLPILVLVMDIIIIVGGPotential 4-gene operon; 1st (SPNA45_00935) encodes pyridoxal-dependent decarboxylase, 3rd (SPNA45_00937) encodes long-chain fatty acid CoA ligase, 4th (SPNA45_00938) encodes macrolide efflux ABC transporter permease
A66dNZ_LN847353.1Yes (96)A66_00963YesMKKISKFLPILVLVMDIIIIPotential 3-gene operon followed by 2-gene operon very similar in organization to SPNA45_01354
Yes (87)A66_01135YesMKKISKFLPILVLVMDIIIIVGGPotential 4-gene operon; 1st gene downstream (A66_01134) encodes siderophore biosynthesis decarboxylase, 2nd encodes nonribosomal peptide synthetase, 4th encodes membrane-spanning ABC transporter
NT_110_58NZ_CP007593.1Yes (95)SpnNT_00981YesMKKISKFLPILVLVMDIIIIPotential 7-gene operon; 1st gene (SpnNT_00982) encodes LanM, 2nd encodes lactococcin G-processing and transport ATP-binding protein (LagD), 3rd (SpnNT_00984) encodes alpha-hemolysin translocation ATP-binding protein (HlyB)
ATCC 700669NC_011900.1Yes (94)SPN23F09790YesMKKISKFLPILVLVMDIIIIPotential 2-gene operon followed by 3-gene operon; 1st gene (SPN23F_09810) encodes lantibiotic export protein, 3rd (SPN23F_09830) encodes lantibiotic transport ATP-binding protein
P1031NC_012467.1Yes (91)SPP_1060YesMKKISKFFPILMLVMDIIIIVGGPotential 7-gene operon; 1st gene (SPP_1062) encodes NAD-binding domain of 6-phosphogluconate dehydrogenase family, 6th (SPP_1067) encodes UDP-glucose 4-epimerase (GalE1), 7th (SPP_1068) encodes biotin carboxylase
NCTC7465NZ_LN831051.1Yes (91)ERS445053_01678YesMKKISKFFPILMLVMDIIIIVGGPotential 7-gene operon very similar to SPP_1060
gamPNI0373NC_018630.1Yes (91)HMPREF1038_01125YesMKKISKFFPILMLVMDIIIIVGGPotential 7-gene operon very similar to SPP_1060
AP200NC_014494.1Yes (89)SPAP_1144YesMKKISKFFPILMLVMDIIIIVGGPotential 2-gene operon with unknown function; immediately downstream there are 3 other operons composed of 4, 2, and 3 genes, respectively
G54dNC_011072.1Yes (88)SPG_0976YesMKKISKFFPILMLVMDIIIIVGGPotential 2-gene operon followed by 7-gene operon; last 2 genes (SPG_0984, SPG_0985) are ABC transporter permeases; SPG_0982 encodes UDP-glucose 4-epimerase
Yes (34)SPG_1268YesMKKYYQIFLLLFDIIIIGLYQPotential 4-gene operon with unknown function
670–6 BNC_014498.1Yes (36)SP670_1149YesMKKYYQIFLLLFDIIIIGLYQPotential 4 operons composed of 2, 1, 2, and 2 genes, respectively; all encode hypothetical proteins except 2nd gene (SP670_1147), which encodes PcfD (bacteriocin ABC-type transporter)
INV200NC_017593.1No
CGSP14NC_010582.1No
TIGR4NC_003028.3No
70585NC_012468.1No
Taiwan19F-14NC_012469.1No
Hungary19A-6NC_010380.1No
TCH8431/19ANC_014251.1No
OXC141NC_017592.1No
INV104NC_017591.1No
SPN034156NC_021006.1No
SPN034183NC_021028.1No
SPN994038NC_021026.1No
SPN994039NC_021005.1No
SPN032672NC_021003.1No
SPN033038NC_021004.1No
ST556NC_017769.2No
SP49NZ_CP018136.1No
SP61NZ_CP018137.1No
SP64NZ_CP018138.1No
SWU02NZ_CP018347.1No
  • a Hits with >30% identity with Rgg0939 in an NCBI tBLASTn search were considered.

  • b NCBI accession numbers for protein coding genes.

  • c Operon prediction was conducted with DOOR (35).

  • d Strains G54, A66, and SPNA45 have two proteins similar to Rgg0939.