TABLE 3 

Plasmids used in this study

Plasmid no.NameRelevant feature(s)Reference/source
pND294CIp10CaURA3 RPS1 integrative plasmid42
pND500CIp-His1CaHIS1 RPS1 integrative plasmid16
pND383CIp-Arg4CaArg4 RPS1 integrative plasmidThis study
pND442YPB1-ADHpCaADH1 promoter/URA3/C. albicans ARS35
pND354CIpHis1-PADH1 yEmRFPyEmRFP driven by CaADH1 promoter in CIp-His16
pND425CIpArg4-PTEF CaCas9Codon-optimized Cas9 driven by AgTEF1 promoter in CIp-ArgThis study
pND459YPB-PADH1 HH gRFP HDVPADH1HH gRFP HDV in YPBThis study
pND465YPB-PADH1 HH gLeu2 HDVPADH1HH gLeu2 HDV in YPBThis study
pND468YPB-PADH1 tA gRFP HDVPADH1tRNA gRFP HDV in YPBThis study
pND479YPB-PtRNA gRFP HDVPtRNA gRFP HDV in YPBThis study
pND474pV1090PSNR52-gRNA/SATR integrative plasmid1
pND476pV1090-gRFPPSNR52-gRFP in pV1090This study
pND489pV1025CaCas9/SAT flipper ENO1 integrative plasmid1
pND486CIp10-PADH1 HH gRFP HDVPADH1HH sgRFP HDV in CIp10This study
pND482CIp10-PADH1 tA gRFP HDVPADH1tRNA sgRFP HDV in CIp10This study
pND483CIp10-PtRNA gRFP HDVPtRNA sgRFP HDV in CIp10This study
pND484CIp10-PSNR52 gRFPPSNR52 sgRFP in CIp10This study
pND494CIp10-PADH1 tA SapI HDVCIP10-based cloning vector for ligation of gRNA PADH1tA-SapI2× HDV in CIp10This study
pND499CIp10-PADH1 tA gLEU2 HDVPADH1tRNA sgLeu2 HDV in CIp10This study
pND501CIp-dpl-PADH1 tA SapI HDVpND494 but contains ura3-dpl200 instead of URA3This study