|Transformationa||Total no. of transformantsb||% of colonies:||% of transformants:|
|(−) Cas9 (EPC1), (+) vector (−gRFP)||~1,000||100||0||0||100||0|
|(−) Cas9 (EPC1), (+) gRFP||~1,000||100||0||0||99||0|
|(−) Cas9 (EPC1), (+) gRFP, (+) healing fragment||~1,000||100||0||0||100||0|
|(+) Cas9 (EPC2), (+) vector (−gRFP)||~1,000||100||0||0||100||80|
|(+) Cas9 (EPC2), (+) gRFP||~1,000||8||90||2||30||80|
|(+) Cas9 (EPC2), (+) gRFP, (+) healing fragment||~1,000||8||92||0||97||73|
↵a Transformations were performed with URA3-marked PADH-tRNA-gRFP-HDV plasmid pND482 (+gRFP) or vector lacking gRFP (−gRFP). (−), donor repair fragment absent; (+), donor repair fragment present. The results shown represent an average of 5 separate experiments, in which the percentage of white colonies ranged from 90 to 98%.
↵b Number of uracil prototrophic transformants.
↵c Percentage of uracil prototrophic transformants that were histidine prototrophs.
↵d Percentage of uracil prototrophic transformants that were arginine prototrophs.