TABLE 4 

Strains used in this study

StrainGenotypeReference
BWP17ura3Δ::λ imm434/ura3Δ::λ imm434 his1Δ::hisG/his1Δ::hisG arg4Δ::hisG/arg4Δ::hisG27
EPC1BWP17 RPS1::PADH1 RFP-HIS1 RPS1This study
EPC2BWP17 RPS1::PADH1 RFP-HIS1-RPS1::PTEF1 CaCas9-HA-ARG4-RPS1This study
RJY54BWP17 RPS1::PTEF1 CaCas9-HA-ARG4-RPS1This study
HNY25BWP17 and leu2Δ/leu2ΔThis study
HNY30BWP17 eno1Δ::CaCas9This study
HNY31BWP17 eno1Δ::CaCas9 leu2Δ/leu2ΔThis study