International K. kingae isolates are represented by four capsule types. (A) A representative agarose gel of the multiplex capsule-typing PCR approach is shown. The type a amplicon is ~2,000 bp (lane 1), the type b amplicon is ~1,500 bp (lane 2), the type c amplicon is ~1,000 bp (lane 3), and the type d amplicon is ~500 bp (lane 4). The genomic DNA PCR template sources are as follows: lane 1, KK01 (type a control); lane 2, KK58 (type b control); lane 3, KK60 (type c control); lane 4, BB270 (type d control); lane 5, DNA ladder; lane 6, 16RZ2819K (type a); lane 7, 16SB9163M (type a); lane 8, ATCC 23330 (type c); lane 9, ATCC 23331 (type a); lane 10, ATCC 23332 (type a); lane 11, SW353 (type b); lane 12, SW628 (type b); lane 13, SW268 (type a); lane 14, AUS 01 (type b); lane 15, KK194 (type d).
Isolate CAN8 is nonencapsulated. Mild-acid surface extracts from capsule type a strain KK01 (lane 1), type b strain KK58 (lane 2), type c strain KK60 (lane 3), type d strain BB270 (lane 4), and isolate CAN8 (lane 5) were analyzed following SDS-PAGE and alcian blue staining. The lack of alcian blue-stained material in the extract of CAN8 confirms that this isolate is nonencapsulated.